TRIm72 Protein, Human

TRIm72 Protein, Human is the recombinant human-derived TRIm72, expressed by E. coli , with tag Free labeled tag. ,

TRIM72 is a muscle-specific E3 ubiquitin-protein ligase that plays a central role in cell membrane repair by nucleating the assembly of the repair machinery at injury sites (PubMed:36944613). Its ubiquitination activity is mediated by E2 ubiquitin-conjugating enzymes UBE2D1, UBE2D2, and UBE2D3 (By similarity). It acts as a sensor of oxidation: upon membrane damage, entry of extracellular oxidative environment results in disulfide bond formation and homooligomerization at the injury site (By similarity). This oligomerization acts as a nucleation site for recruitment of TRIM72-containing vesicles to the injury site, leading to membrane patch formation (By similarity). It likely functions upstream of the Ca²⁺-dependent membrane resealing process (By similarity). Required for transport of DYSF to sites of cell injury during repair patch formation (By similarity). Regulates membrane budding and exocytosis (By similarity). May be involved in modulating the mobility of KCNB1-containing endocytic vesicles (By similarity).

Human

E. coli

Tag Free

Q6ZMU5-1 (S2-A477)

493829

Partial

Approximately 52.8 kDa

Greater than 80% as determined by reducing SDS-PAGE

Solution.

Supplied as a 0.22 μm filtered solution of 50 mM HEPES , pH 7.5, 200 mM NaCl, 20% glycerol, 1 mM DTT.

<1 EU/μg, determined by LAL method.

Please use rapid thawing with running water to thaw the protein.

Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping with dry ice.