TPO/Thrombopoietin Protein, Mouse (335a.a, HEK293, His)

TPO/Thrombopoietin Protein, a lineage-specific cytokine, crucially regulates megakaryocyte proliferation and maturation, acting in the late stages of development from committed progenitor cells. Recognized as a major physiological regulator, TPO significantly influences circulating platelets, emphasizing its importance in the intricate process of platelet formation. TPO/Thrombopoietin Protein, Mouse (HEK293, His) is the recombinant mouse-derived TPO/Thrombopoietin protein, expressed by HEK293 , with N-6*His labeled tag.

Thrombopoietin (TPO), a lineage-specific cytokine, plays a crucial role in regulating the proliferation and maturation of megakaryocytes, acting at a late stage of their development from committed progenitor cells. Functioning as a key factor in megakaryopoiesis, TPO is recognized as a major physiological regulator of circulating platelets. Its influence on the later stages of megakaryocyte development underscores its significance in the intricate process of platelet formation.

Measured in a cell proliferation assay using MO7e human megakaryocytic leukemic cells. The ED₅₀ for this effect is 0.9757 ng/mL, corresponding to a specific activity is 1.25×10⁶ units/mg.

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  Measured in a cell proliferation assay using MO7e human megakaryocytic leukemic cells. The ED₅₀ for this effect is 0.9757 ng/mL, corresponding to a specific activity is 1.25×10⁶ units/mg.

Mouse

HEK293

N-6*His

P40226-1 (S22-T356)

21832  [NCBI]

Full Length of Isoform-1 Mature Protein

SPVAPACDPRLLNKLLRDSHLLHSRLSQCPDVDPLSIPVLLPAVDFSLGEWKTQTEQSKAQDILGAVSLLLEGVMAARGQLEPSCLSSLLGQLSGQVRLLLGALQGLLGTQLPLQGRTTAHKDPNALFLSLQQLLRGKVRFLLLVEGPTLCVRRTLPTTAVPSSTSQLLTLNKFPNRTSGLLETNFSVTARTAGPGLLSRLQGFRVKITPGQLNQTSRSPVQISGYLNRTHGPVNGTHGLFAGTSLQTLEASDISPGAFNKGSLAFNLQGGLPPSPSLAPDGHTPFPPSPALPTTHGSPPQLHPLFPDPSTTMPNSTAPHPVTMYPHPRNLSQET

80-110 kDa

Greater than 95% as determined by reducing SDS-PAGE.

Lyophilized powder.

Lyophilized from a 0.22 μm filtered solution of PBS, 2 mM EDTA, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US;may vary elsewhere.