TARDBP Protein, Human (His)

TARDBP protein is an RNA-binding factor with RRM1 and RRM2 that binds GU repeats and regulates RNA splicing in long introns and 3'UTRs.It affects proteins critical for neuronal survival and neurodegenerative diseases.TARDBP Protein, Human (P.pastoris, His) is the recombinant human-derived TARDBP protein, expressed by P.pastoris , with N-6*His labeled tag.

The TARDBP protein, an RNA-binding factor, plays a multifaceted role in various steps of RNA biogenesis and processing. Utilizing its two RNA recognition motifs, RRM1 and RRM2, TARDBP preferentially binds GU-repeats on RNA molecules, predominantly localized within long introns and the 3'UTR of mRNAs. This binding activity regulates the splicing of numerous non-coding and protein-coding RNAs, impacting proteins crucial for neuronal survival and those relevant to neurodegenerative diseases. TARDBP also contributes to mitochondrial homeostasis by regulating the processing of mitochondrial transcripts and modulates mRNA stability by recruiting CNOT7/CAF1 deadenylase to mRNA 3'UTR, leading to poly(A) tail deadenylation. In response to oxidative insult, TARDBP associates with stalled ribosomes in stress granules, contributing to cell survival. Moreover, it participates in skeletal muscle formation and regeneration by forming cytoplasmic myo-granules and binding mRNAs encoding sarcomeric proteins. Additionally, TARDBP is involved in the maintenance of circadian clock periodicity by stabilizing CRY1 and CRY2 proteins in a FBXL3-dependent manner and negatively regulates the expression of CDK6. Interactions with various proteins, including BRDT, ATXN2, MATR3, UBQLN2, HNRNPA2B1, ZNF106, CNOT7/CAF1, CRY2, and PPIA/CYPA, highlight the diverse roles of TARDBP in RNA regulation and cellular processes.

Measured by its binding ability in a functional ELISA.Immobilized Human TDP-43, His Tag at 1 μg/mL (100 μL/well) can bind Anti-TDP43 Antibody.The bioactivity was comparable to standard batch.

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  Immobilized Human TARDBP at 1 μg/mL (100 μL/well) can bind Anti-TARDBP antibody. The ED₅₀ for this effect is 161.8 ng/mL.

Human

E. coli

N-His

Q13148-1 (M1-M414)

23435  [NCBI]

Full Length

MSEYIRVTEDENDEPIEIPSEDDGTVLLSTVTAQFPGACGLRYRNPVSQCMRGVRLVEGILHAPDAGWGNLVYVVNYPKDNKRKMDETDASSAVKVKRAVQKTSDLIVLGLPWKTTEQDLKEYFSTFGEVLMVQVKKDLKTGHSKGFGFVRFTEYETQVKVMSQRHMIDGRWCDCKLPNSKQSQDEPLRSRKVFVGRCTEDMTEDELREFFSQYGDVMDVFIPKPFRAFAFVTFADDQIAQSLCGEDLIIKGISVHISNAEPKHNSNRQLERSGRFGGNPGGFGNQGGFGNSRGGGAGLGNNQGSNMGGGMNFGAFSINPAMMAAAQAALQSSWGMMGMLASQQNQSGPSGNNQNQGNMQREPNQAFGSGNNSYSGSNSGAAIGWGSASNAGSGSGFNGGFGSSMDSKSSGWGM

Approximately 54-56 kDa, based on SDS-PAGE under reducing conditions.

Greater than 90% as determined by reducing SDS-PAGE.

Solution.

Supplied as a 0.22 μm filtered solution of 50 mM HEPES, 500 mM NaCl, 0.4 M Arginine, pH 8.0 with 20% glycerol as protectant.

<1 EU/μg, determined by LAL method.

N/A.

Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping with dry ice.