1. Recombinant Proteins
  2. Enzymes & Regulators
  3. Transferases (EC 2)
  4. TAG-72 Protein, Canine (His)

TAG-72 Protein, Canine (His) is the recombinant canine-derived TAG-72 protein, expressed by E. coli, with C-His tag.

For research use only. We do not sell to patients.

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Top Publications Citing Use of Products
  • Biological Activity

  • Technical Parameters

  • Properties

  • Documentation

Description

TAG-72 Protein, Canine (His) is the recombinant canine-derived TAG-72 protein, expressed by E. coli, with C-His tag.

Background

TAG-72 is the catalytic subunit of the mRNA-capping methyltransferase RNMT:RAMAC complex, which methylates the N7 position of the guanosine added to the 5'-cap structure of mRNAs. It binds RNA containing a 5'-terminal GpppC.

Species

Canine

Source

E. coli

Tag

C-8*His

Accession

XP_005615459 (M1-Q478)

Gene ID

476200

Protein Length

Full Length

Synonyms
TAG-72
Molecular Weight

Approximately 56.19 kDa.

Purity

Greater than 95% as determined by Bis-Tris PAGE.

Appearance

Lyophilized powder

Formulation

Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4. Normally 8% trehalose is added as protectant before lyophilization.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O.

Storage & Stability

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US; may vary elsewhere.

Documentation

TAG-72 Protein, Canine (His) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • Reconstitution Calculator

  • Dilution Calculator

  • Specific Activity Calculator

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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Product Name:
TAG-72 Protein, Canine (His)
Cat. No.:
HY-P703820
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