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  4. STEAP1 Protein, Human (HEK293, His, Twin-Strep)

STEAP1 Protein, Human (HEK293, His, Twin-Strep)

Cat. No.: HY-P704159
Handling Instructions Technical Support

STEAP1 protein lacks the necessary binding sites for the electron-donating substrate NADPH, therefore it does not function as a metalloreductase. However, when fused to the NADPH-binding domain of STEAP4, it can facilitate the reduction of Fe(3+). STEAP1 Protein, Human (HEK293, His, Twin-Strep) is the recombinant human-derived STEAP1 protein, expressed by HEK293, with C-His and N-Twin-Strep labeled tag.

For research use only. We do not sell to patients.

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Description

STEAP1 protein lacks the necessary binding sites for the electron-donating substrate NADPH, therefore it does not function as a metalloreductase. However, when fused to the NADPH-binding domain of STEAP4, it can facilitate the reduction of Fe(3+). STEAP1 Protein, Human (HEK293, His, Twin-Strep) is the recombinant human-derived STEAP1 protein, expressed by HEK293, with C-His and N-Twin-Strep labeled tag.
Background

STEAP1 Protein, a member of the six-transmembrane epithelial antigen of the prostate family, is involved in iron regulation and tumor progression. It is overexpressed in various cancers and contributes to cell proliferation and metastasis. The potential of STEAP1 Protein as a therapeutic target and its role in cancer development make it a subject of interest in oncology research.
Species

Human
Source

HEK293
Tag

C-His;N-Twin-Strep
Accession

Q9UHE8 (E2-L339)
Gene ID

26872
Molecular Construction
N-term
Twin-Strep
STEAP1 (E2-L339)
Accession # Q9UHE8-1
His
C-term
Protein Length

Partial
Synonyms
STEAP1, PRSS24, STEAP
AA Sequence

ESRKDITNQEELWKMKPRRNLEEDDYLHKDTGETSMLKRPVLLHLHQTAHADEFDCPSELQHTQELFPQWHLPIKIAAIIASLTFLYTLLREVIHPLATSHQQYFYKIPILVINKVLPMVSITLLALVYLPGVIAAIVQLHNGTKYKKFPHWLDKWMLTRKQFGLLSFFFAVLHAIYSLSYPMRRSYRYKLLNWAYQQVQQNKEDAWIEHDVWRMEIYVSLGIVGLAILALLAVTSIPSVSDSLTWREFHYIQSKLGIVSLLLGTIHALIFAWNKWIDIKQFVWYTPPTFMIAVFLPIVVLIFKSILFLPCLRKKILKIRHGWEDVTKINKTEICSQL

Predicted Molecular Mass
44.7 kDa
Molecular Weight

Approximately 40-42 kDa & 80 kDa, based on SDS-PAGE under reducing conditions, due to the glycosylation.
Glycosylation
Yes
Purity

Greater than 85% as determined by reducing SDS-PAGE.
Endotoxin Level

<1 EU/μg, determined by LAL method.
Documentation
Handling Instructions (2659 KB)

STEAP1 Protein, Human (HEK293, His, Twin-Strep) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • Reconstitution Calculator

  • Dilution Calculator

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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STEAP1 Protein, Human (HEK293, His, Twin-Strep)
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