Staphylokinase Protein, Staphylococcus phage (His)

Staphylococcal kinase protein is a potent plasminogen activator that converts plasminogen into active plasmin. In addition to activation, staphylococcal kinase forms a 1:1 complex with plasmin, enabling activated plasmin to catalyze the conversion of other plasminogen molecules. Staphylokinase Protein, Staphylococcus phage (His) is the recombinant mouse-derived Staphylokinase protein, expressed by E. coli , with N-6*His labeled tag. The total length of Staphylokinase Protein, Staphylococcus phage (His) is 136 a.a., with molecular weight of ~17 kDa.

Staphylokinase Protein emerges as a potent plasminogen activator, facilitating the conversion of plasminogen into its active form, plasmin. This enzymatic process is pivotal in the fibrinolytic system, as Staphylokinase not only serves as an activator but also forms a 1:1 complex with plasmin. In this complex, plasmin is activated and gains the ability to further catalyze the conversion of additional plasminogen molecules. The intricate interplay between Staphylokinase and plasmin underscores the protein's role as a key regulator in the fibrinolytic cascade, contributing to the dissolution of blood clots and highlighting its potential therapeutic applications in promoting clot resolution.

Fully biologically active when compared to standard. The specific activity determined by using chromogenic substrate s-2251 that incubate at 37°C for 10 minutes is 5.30 × 10⁴ IU/mg.

Others

E. coli

N-6*His

P15240 (S28-K163)

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SSSFDKGKYKKGDDASYFEPTGPYLMVNVTGVDGKRNELLSPRYVEFPIKPGTTLTKEKIEYYVEWALDATAYKEFRVVELDPSAKIEVTYYDKNKKKEETKSFPITEKGFVVPDLSEHIKNPGFNLITKVVIEKK

Approximately 18 kDa

Greater than 95% as determined by reducing SDS-PAGE

Lyophilized powder

Lyophilized from a 0.22 μm solution of 50 mM HEPES, 150 mM NaCl, pH 6.8, 8% trehalose.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.