Src Protein, Human (sf9, His, Flag)

Src is a non-receptor protein tyrosine kinase that centralizes multiple cellular processes triggered by various receptors. It controls gene transcription, immune response, adhesion, cell cycle, apoptosis, migration and transformation. Src Protein, Human (sf9, His, Flag) is the recombinant human-derived Src, expressed by Sf9 insect cells , with His, Flag labeled tag. ,

Src, a non-receptor protein tyrosine kinase, emerges as a central player in an array of cellular processes triggered by the engagement of diverse receptors, including immune response receptors, integrins, receptor protein tyrosine kinases, G protein-coupled receptors, and cytokine receptors. It orchestrates signaling pathways governing gene transcription, immune responses, cell adhesion, cell cycle progression, apoptosis, migration, and cellular transformation. While functional redundancy within the SRC kinase family complicates the identification of specific roles, SRC appears to be a primary kinase activated upon receptor engagement, influencing the activation of other protein tyrosine kinase (PTK) families. SRC is implicated in the regulation of cytoskeletal organization by phosphorylating substrates like AFAP1 and cortactin (CTTN). Its involvement extends to cell-cell and cell-matrix junctions, where it phosphorylates components such as beta-catenin (CTNNB1), delta-catenin (CTNND1), plakoglobin (JUP), and connexin-43 (GJA1). Additionally, SRC participates in PDGF-mediated tyrosine phosphorylation of STAT1 and STAT3, the RAS pathway, EGF-mediated calcium-activated chloride channel activation, and beta-arrestin desensitization. It also plays a crucial role in EGFR internalization, CDK20/MAPK3 mitogen-activated protein kinase cascade stimulation, and osteoclastic bone resorption. SRC's multifaceted activities highlight its intricate role in cellular regulation and signal transduction.

Human

Sf9 insect cells

N-His;N-Flag

P12931-1 (G2-L536)

6714

Full Length of Isoform-1 Mature Protein

Greater than 90% as determined by reducing SDS-PAGE.

Solution.

Supplied as a 0.2 μm filtered solution of 50 mM HEPES, 150 mM NaCl, 1 mM ATP, 5 mM MgCl₂, pH 7.5.

<1 EU/μg, determined by LAL method.

Please use rapid thawing with running water to thaw the protein.

Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping with dry ice.