2. Recombinant Proteins
  3. Enzymes & Regulators
  4. SMAD5 Protein, Human (GST)

SMAD5 Protein, Human (GST) is the recombinant human-derived SMAD5, expressed by E. coli , with GST labeled tag. ,

For research use only. We do not sell to patients.

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Description

SMAD5 Protein, Human (GST) is the recombinant human-derived SMAD5, expressed by E. coli , with GST labeled tag. ,
Background

Smad1/5/9 is a transcriptional regulator involved in various cellular processes such as embryonic development, cell differentiation, angiogenesis, and tissue homeostasis. Upon BMP ligand binding to cell surface receptors, it is phosphorylated by activated type I BMP receptors (BMPRIs) and associates with SMAD4 to form a heteromeric complex that translocates into the nucleus, functioning as a transcription factor. The hetero-trimeric complex recognizes cis-regulatory elements containing Smad Binding Elements (SBEs) to modulate signaling network outcomes. Non-phosphorylated SMAD5 plays a cytoplasmic role in energy metabolism regulation by promoting mitochondrial respiration and glycolysis in response to cytoplasmic pH changes. Mechanistically, it interacts with hexokinase 1 (HK1) to accelerate glycolysis.
Species

Human
Source

E. coli
Tag

GST
Accession

Q99717 (M1-S465)
Gene ID

4090
Protein Length

Full Length
Synonyms
MADH5
Molecular Weight

Approximately 82 kDa, based on SDS-PAGE under reducing conditions.
Purity

Greater than 80% as determined by reducing SDS-PAGE
Documentation
Handling Instructions (2659 KB)

SMAD5 Protein, Human (GST) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • Reconstitution Calculator

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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