2. Recombinant Proteins
  3. Enzymes & Regulators
  4. Transferases (EC 2)
  5. SCP2 Protein, Human (GST)

SCP2 Protein, Human (GST) is the recombinant human-derived SCP2 protein, expressed by E. coli, with N-GST tag.

For research use only. We do not sell to patients.

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Description

SCP2 Protein, Human (GST) is the recombinant human-derived SCP2 protein, expressed by E. coli, with N-GST tag.
Background

SCP2 plays a crucial role in the peroxisomal oxidation of branched-chain fatty acids (PubMed:10706581). It catalyzes the last step of the peroxisomal beta-oxidation of branched chain fatty acids and the side chain of the bile acid intermediates di- and trihydroxycoprostanic acids (DHCA and THCA) (PubMed:10706581). The enzyme is also active with medium and long straight chain 3-oxoacyl-CoAs. In vitro, it stimulates the microsomal conversion of 7-dehydrocholesterol to cholesterol and transfers phosphatidylcholine and 7-dehydrocholesterol between membranes (By similarity). The SCP2 and SCPx isoforms cooperate in peroxisomal oxidation of certain naturally occurring tetramethyl-branched fatty acyl-CoAs (By similarity).
Species

Human
Source

E. coli
Tag

N-GST
Accession

P22307-2 (M1-L143)
Gene ID

6342
Molecular Construction
N-term
GST
SCP2 (M1-L143)
Accession # P22307-2
C-term
Protein Length

Full Length of Isoform-2
Synonyms
DKFZp686C12188; DKFZp686D11188; NLTP; NLTP_HUMAN; Non-specific lipid-transfer protein; Nonspecific lipid transfer protein; NSL TP; NSL-TP; Propanoyl CoA C acyltransferase; Propanoyl-CoA C-acyltransferase; SCP 2; SCP chi ; SCP X; SCP-2; SCP-chi; SCP-X; SCP2; SCPchi ; SCPX; Sterol carrier protein 2; Sterol carrier protein X
AA Sequence

MGFPEAASSFRTHQIEAVPTSSASDGFKANLVFKEIEKKLEEEGEQFVKKIGGIFAFKVKDGPGGKEATWVVDVKNGKGSVLPNSDKKADCTITMADSDFLALMTGKMNPQSAFFQGKLKITGNMGLAMKLQNLQLQPGNAKL

Predicted Molecular Mass
42.4 kDa
Purity

Greater than 95% as determined by reducing SDS-PAGE.
Endotoxin Level

<1 EU/μg, determined by LAL method.
Documentation
Handling Instructions (2659 KB)

SCP2 Protein, Human (GST) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • Reconstitution Calculator

  • Dilution Calculator

  • Specific Activity Calculator

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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