SAMHD1 Protein, Human (sf9, His, Strep)

SAMHD1 Protein, Human (sf9, His, Strep) is the recombinant human-derived SAMHD1, expressed by sf9 insect cells , with Strep, His labeled tag. ,

SAMHD1 is a multifunctional protein that serves as both a host restriction factor in antiviral defense and a regulator of DNA end resection at stalled replication forks. Its deoxynucleoside triphosphate (dNTPase) activity restricts viral infections like HIV-1 by depleting cellular dNTP pools below the threshold required for retroviral reverse transcription, thereby blocking early-stage replication in dendritic and myeloid cells, while also suppressing LINE-1 retrotransposon activity. This restriction is counteracted by HIV-2's Vpx protein. The dNTPase activity additionally maintains DNA precursor homeostasis by regulating dNTP pools. Phosphorylation at Thr-592 acts as a molecular switch, inhibiting dNTPase-dependent antiviral functions while promoting DNA end resection at stalled forks. During S phase, SAMHD1 stimulates MRE11 exonuclease activity to process gapped or reversed replication forks, facilitating replication restart and preventing chronic inflammation by averting type I interferon induction (a function independent of dNTPase activity). By similarity, it enhances immunoglobulin hypermutation in B-lymphocytes via transversion mutations.

Human

Sf9 insect cells

N-His;N-Strep

Q9Y3Z3-1 (Q2-M626)

25939

Partial

Approximately 75.3 kDa

Greater than 80% as determined by reducing SDS-PAGE

Solution.

Supplied as a 0.22 μm filtered solution of 50 mM Tris-HCl, pH 7.5, 200 mM NaCl, 20% glycerol, 1 mM DTT.
Note: For SPR assay, please replace the buffer. Primary amine components (e.g., Tris, imidazole) can affect protein-coupled chips.

<1 EU/μg, determined by LAL method.

Please use rapid thawing with running water to thaw the protein.

Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping with dry ice.