RVFV-L Protein (His)

RVFV-L Protein (His) is the recombinant Virus-derived RVFV-L protein, expressed by E. coli, with C-His labeled tag.

RVFV-L is an RNA-dependent RNA polymerase responsible for replicating and transcribing the viral RNA genome using antigenomic RNA as an intermediate. During transcription, it synthesizes subgenomic RNAs and ensures their capping via a cap-snatching mechanism, which involves endonuclease activity cleaving host capped pre-mRNAs. These short capped RNAs are then used as primers for viral transcription. The 3'-ends of subgenomic mRNA molecules are not polyadenylated. During replication, the polymerase binds the 5' and 3' vRNA extremities at distinct sites, triggering significant conformational changes in both the polymerase and vRNA to initiate active RNA synthesis. Since the same enzyme is used for both transcription and replication, these mechanisms must be tightly coordinated.

Virus

E. coli

C-His

AEB20483.1 (M1-F250)

/

Partial

Greater than 95% as determined by reducing SDS-PAGE.

<1 EU/μg, determined by LAL method.