RNF4 Protein, Human (Biotinylated)

RNF4 protein is an E3 ubiquitin protein ligase that can uniquely bind and ubiquitinate polyubiquitination chains, mediating "Lys-6", "Lys-11", "Lys-48" and "Lys- 63"-linked polyubiquitination to promote proteasomal degradation. Its multiple substrates, including PML and PEA3, regulate various cellular processes. RNF4 Protein, Human (Biotinylated) is the recombinant human-derived RNF4, expressed by E. coli , with tag Free labeled tag. ,

RNF4 Protein operates as an E3 ubiquitin-protein ligase with a distinctive ability to bind polysumoylated chains covalently attached to proteins, facilitating the 'Lys-6', 'Lys-11', 'Lys-48', and 'Lys-63'-linked polyubiquitination of its substrates. These substrates are subsequently targeted for proteasomal degradation, contributing to the regulation of various cellular processes. RNF4 plays a role in the degradation of proteins such as PML and the transcriptional activator PEA3. In the context of chromosome dynamics, it influences kinetochore assembly, specifically targeting polysumoylated CENPI for proteasomal degradation and thereby impacting chromosome alignment and spindle assembly. Furthermore, RNF4 is implicated in cellular responses to hypoxia and heat shock, facilitating the degradation of EPAS1 and PARP1, respectively. Additionally, RNF4 may interact with DNA/nucleosomes, suggesting a potential direct involvement in transcriptional regulation, including the enhancement of basal transcription and steroid receptor-mediated transcriptional activation. Notably, it catalyzes the ubiquitination of sumoylated PARP1 in response to PARP1 trapping to chromatin, leading to the removal of PARP1 from chromatin facilitated by VCP/p97.

Human

E. coli

Tag Free

P78317-1 (S2-I190)

6047

Partial

Greater than 95% as determined by reducing SDS-PAGE

Solution.

Supplied as a 0.22 μm filtered solution of 50 mM HEPES (pH 7.5), 200mM NaCl, 20% glycerol, 1mM DTT.

<1 EU/μg, determined by LAL method.

Please use rapid thawing with running water to thaw the protein.

Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping with dry ice.