2. Recombinant Proteins
  3. Enzymes & Regulators
  4. Oxidoreductases (EC 1)
  5. rLuc Protein, Human (His, Strep)

rLuc Protein, Human (His, Strep) is the recombinant human-derived rLuc, expressed by E. coli , with Strep, His labeled tag. ,

For research use only. We do not sell to patients.

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  • Biological Activity

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Description

rLuc Protein, Human (His, Strep) is the recombinant human-derived rLuc, expressed by E. coli , with Strep, His labeled tag. ,
Background

Renilla Luciferase is an enzyme that, upon binding its substrate, catalyzes an oxygenation reaction, producing a highly unstable hydroperoxide that cyclizes into a dioxetanone structure. The dioxetanone then collapses, releasing a CO(2) molecule. The spontaneous breakdown of the dioxetanone releases approximately 50 kcal/mol of energy, which is required to generate the excited state of the coelenteramide product (the singlet form of the monoanion). In vivo, the product undergoes nonradiative energy transfer to an accessory protein, green fluorescent protein (GFP), resulting in green bioluminescence. In vitro, in the absence of GFP, the product emits blue light.
Species

Human
Source

E. coli
Tag

Strep;His
Accession

P27652 (M1-Q311)
Gene ID

/
Protein Length

Full Length
Synonyms
Coelenterazine h 2-monooxygenase; Renilla-luciferin 2-monooxygenase; Renilla-type luciferase; Renilla reniformis; Sea pansy; Decarboxylase; Lyase; Monooxygenase; Oxidoreductase; Photoprotein; 1.13.12.5
Predicted Molecular Mass
39.2 kDa
Endotoxin Level

<1 EU/μg, determined by LAL method.
Documentation
Handling Instructions (2659 KB)

rLuc Protein, Human (His, Strep) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • Reconstitution Calculator

  • Dilution Calculator

  • Specific Activity Calculator

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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rLuc Protein, Human (His, Strep)
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