2. Recombinant Proteins
  3. Viral Proteins Others
  4. pp1a Protein, AIBV (His)

pp1a Protein, AIBV (His) is the recombinant virus-derived pp1a protein, expressed by E. coli, with His tag.

For research use only. We do not sell to patients.

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Top Publications Citing Use of Products

  • Biological Activity

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Description

pp1a Protein, AIBV (His) is the recombinant virus-derived pp1a protein, expressed by E. coli, with His tag.
Background

pp1a is a multifunctional protein involved in the transcription and replication of viral RNAs. It contains the proteinases responsible for the cleavages of the polyprotein.
Species

Virus
Source

E. coli
Tag

His
Accession

P0C6V5 (G2783-Q3088)
Gene ID

/
Protein Length

Partial
Synonyms
Replicase polyprotein 1a; ORF1a polyprotein; Avian infectious bronchitis virus (strain M41); IBV; pp1a; Hydrolase; Lyase; Protease; RNA-binding; Thiol protease
Predicted Molecular Mass
35.7 kDa
Purity

Greater than 80% as determined by reducing SDS-PAGE
Endotoxin Level

<1 EU/μg, determined by LAL method.
Documentation
Handling Instructions (2659 KB)

pp1a Protein, AIBV (His) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • Reconstitution Calculator

  • Dilution Calculator

  • Specific Activity Calculator

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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pp1a Protein, AIBV (His)
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