RANKL/TNFSF11 Protein, Human (HEK293, His)

RANKL/TNFSF11 Protein, Human (HEK293, His) is the recombinant human-derived RANKL/TNFSF11 protein, expressed by HEK293, with N-His tag.

RANKL/TNFSF11 is a cytokine that binds to TNFRSF11B/OPG and TNFRSF11A/RANK. It acts as an osteoclast differentiation and activation factor, enhances the ability of dendritic cells to stimulate naive T-cell proliferation, and may be a key regulator of T-cell–dendritic cell interactions and T-cell-dependent immune responses. It also likely contributes to increased bone resorption in humoral hypercalcemia of malignancy. By activating multiple signaling pathways in osteoclast precursor cells—particularly through inducing prolonged intracellular Ca ²⁺ oscillations that activate NFATC1 (leading to its nuclear translocation and induction of osteoclast-specific gene transcription)—RANKL/TNFSF11 promotes osteoclastogenesis. During osteoclast differentiation, it also triggers TMEM64- and ATP2A2-dependent CREB1 activation and mitochondrial ROS generation, which are essential for proper osteoclast formation (By similarity).

Human

HEK293

N-His

AAC51762.1 (G64-D245)

/

Partial

Approximately 28-35 kDa,based on SDS-PAGE under reducing conditions,due to the glycosylation.

Greater than 90% as determined by reducing SDS-PAGE.

<1 EU/μg, determined by LAL method.