RANKL/TNFSF11 Protein, Mouse (247a.a, HEK293, His)

RANKL/TNFSF11 Protein, Mouse (247a.a, HEK293, His) is the recombinant mouse-derived RANKL/TNFSF11 protein, expressed by HEK293, with C-His tag.

RANKL/TNFSF11 is a cytokine that binds to TNFRSF11B/OPG and TNFRSF11A/RANK. It acts as an osteoclast differentiation and activation factor, enhances the ability of dendritic cells to stimulate naive T-cell proliferation, and may be a key regulator of T-cell–dendritic cell interactions and T-cell-dependent immune responses. It also likely contributes to increased bone resorption in humoral hypercalcemia of malignancy. By activating multiple signaling pathways in osteoclast precursor cells—particularly through inducing prolonged intracellular Ca ²⁺ oscillations that activate NFATC1 (leading to its nuclear translocation and induction of osteoclast-specific gene transcription)—RANKL/TNFSF11 promotes osteoclastogenesis. During osteoclast differentiation, it also triggers TMEM64- and ATP2A2-dependent CREB1 activation and mitochondrial ROS generation, which are essential for proper osteoclast formation (By similarity).

Mouse

HEK293

C-8*His

AAC40113 (Y70-D316)

/

Partial

Approximately 30-40 kDa,based on SDS-PAGE under reducing conditions,due to the glycosylation.

Greater than 95% as determined by Bis-Tris PAGE.

<1 EU/μg, determined by LAL method.