RANK L/TNFSF11 Protein, Human (Biotinylated, HEK293, Fc-Avi)

RANK L/TNFSF11 protein is a cytokine that binds to TNFRSF11B/OPG and TNFRSF11A/RANK and serves as a key regulator of osteoclast differentiation and activation. In addition, it is a factor that enhances the ability of dendritic cells to stimulate naive T cell proliferation, indicating its importance in regulating the interaction between T cells and dendritic cells, as well as its potential role in T cell-dependent immune responses. RANK L/TNFSF11 Protein, Human (Biotinylated, HEK293, Fc-Avi) is the recombinant human-derived RANK L/TNFSF11 protein, expressed by HEK293 , with N-hFc, N-Avi labeled tag. The total length of RANK L/TNFSF11 Protein, Human (Biotinylated, HEK293, Fc-Avi) is 182 a.a., with molecular weight of 55-60 kDa.

RANK L/TNFSF11 protein, a cytokine, binds to TNFRSF11B/OPG and TNFRSF11A/RANK, functioning as a crucial regulator of osteoclast differentiation and activation. Additionally, it serves as a factor that enhances the ability of dendritic cells to stimulate naive T-cell proliferation, suggesting its importance in the regulation of T-cell and dendritic cell interactions, and a potential role in the T-cell-dependent immune response. Moreover, RANK L/TNFSF11 may play a significant role in enhanced bone resorption observed in conditions like humoral hypercalcemia of malignancy. Its induction of osteoclastogenesis involves the activation of multiple signaling pathways in osteoclast precursor cells, with a key mechanism being the induction of long-lasting oscillations in intracellular calcium concentration, leading to the activation of NFATC1. This transcription factor translocates to the nucleus and initiates osteoclast-specific gene transcription, facilitating osteoclast differentiation. During this process, RANK L/TNFSF11, in a TMEM64 and ATP2A2-dependent manner, induces CREB1 activation and mitochondrial ROS generation, crucial for proper osteoclast formation. Existing as a homotrimer, RANK L/TNFSF11 interacts with TNFRSF11B, TNFRSF11A, FBN1, and TNFAIP6, highlighting its versatile interactions in diverse cellular contexts.

Human

HEK293

N-hFc;N-Avi

AAC51762 (G64-D245)

8600  [NCBI]

55-60 kDa

Greater than 90% as determined by reducing SDS-PAGE.

<1 EU/μg, determined by LAL method.