PVRIG Protein, Human (Biotinylated, HEK293, His-Avi)

The PVRIG protein functions as a cell surface receptor for NECTIN2 and may serve as a co-inhibitory receptor capable of inhibiting T-cell receptor-mediated signaling. Upon interaction with NECTIN2, PVRIG inhibits T cell proliferation, suggesting its potential role in regulating immune responses. PVRIG Protein, Human (Biotinylated, HEK293, His-Avi) is the recombinant human-derived PVRIG protein, expressed by HEK293 , with C-Avi, C-His labeled tag. The total length of PVRIG Protein, Human (Biotinylated, HEK293, His-Avi) is 131 a.a., with molecular weight of 25-30 kDa.

PVRIG (PVR-related immunoglobulin domain-containing) protein is identified as a cell surface receptor for NECTIN2, functioning as a potential coinhibitory receptor that dampens T-cell receptor-mediated signals. Upon interacting with NECTIN2, PVRIG exerts its inhibitory effect by suppressing T-cell proliferation. Notably, PVRIG engages in a competitive binding scenario with CD226 for NECTIN2, implying a regulatory role in immune responses where it competes with an activating receptor. This competitive interaction with CD226 underscores PVRIG's involvement in modulating the delicate balance between activating and inhibitory signals during T-cell activation.

Immobilized Biotinylated Human PVRIG His-Avi at 1 μg/mL (100 μL/well) on Streptavidin precoated (0.5 μg/well) plate can bind Human Nectin-2 Fc with a linear range of 0.1-5 ng/mL.

Human

HEK293

C-Avi;C-His

Q6DKI7 (T41-D171)

79037  [NCBI]

25-30 kDa

Greater than 90% as determined by reducing SDS-PAGE.

Lyophilized powder.

Lyophilized a 0.22 μm filtered solution of PBS, pH 7.4. Normally trehalose is added as protectant before lyophilization.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.