2. Recombinant Proteins
  3. Enzymes & Regulators
  4. Transferases (EC 2)
  5. PRPF4B Protein, Human (sf9, GST)

PRPF4B Protein, Human (sf9, GST) is the recombinant human-derived PRPF4B, expressed by Sf9 insect cells , with GST labeled tag. ,

For research use only. We do not sell to patients.

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Description

PRPF4B Protein, Human (sf9, GST) is the recombinant human-derived PRPF4B, expressed by Sf9 insect cells , with GST labeled tag. ,
Background

PRPF4B is a serine/threonine kinase involved in spliceosomal assembly, mitosis, and signaling regulation. It bridges chromatin-mediated transcriptional regulation with pre-mRNA splicing, interacting with and phosphorylating U4/U6-U5 tri-snRNP components PRPF6 and PRPF31 to facilitate spliceosome B complex formation. PRPF4B also regulates transcription and the spindle assembly checkpoint (SAC), associating with U5 snRNP and NCOR1 deacetylase complexes to coordinate splicing with chromatin remodeling. It likely phosphorylates SMARCA4 and NCOR1, as well as SRSF1. During mitosis, PRPF4B localizes to kinetochores and is essential for recruiting/maintaining checkpoint proteins (e.g., MAD1L1, MAD2L1). Additionally, it phosphorylates transcription factors ELK1, KLF13, and nuclear YAP1/WWTR1(TAZ), the latter triggering nuclear exclusion and modulating the Hippo pathway (implicated in tissue growth control).
Species

Human
Source

Sf9 insect cells
Tag

GST
Accession

Q13523 (K551-I1007)
Gene ID

8899
Protein Length

Partial
Synonyms
KIAA0536; PRP4; PRP4B; PRP4H; PRPF4K
Predicted Molecular Mass
82 kDa
Purity

Greater than 90% as determined by reducing SDS-PAGE
Documentation
Handling Instructions (2659 KB)

PRPF4B Protein, Human (sf9, GST) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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