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  4. Prok2 Protein, Mouse (His-B2M)

Prok2 Protein, Mouse (His-B2M) is the recombinant mouse-derived Prok2 protein, expressed by E. coli, with N-B2M & N-6*His tag.

For research use only. We do not sell to patients.

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  • Biological Activity

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Description

Prok2 Protein, Mouse (His-B2M) is the recombinant mouse-derived Prok2 protein, expressed by E. coli, with N-B2M & N-6*His tag.
Background

Prok2 may function as an output molecule from the suprachiasmatic nucleus (SCN) that transmits behavioral circadian rhythm. It could also act locally within the SCN to synchronize output. Additionally, Prok2 potently contracts gastrointestinal (GI) smooth muscle (by similarity).
Species

Mouse
Source

E. coli
Tag

N-B2M;N-6*His
Accession

Q9QXU7 (A27-K128)
Gene ID

50501
Molecular Construction
N-term
B2M
6*His
Prok2 (A27-K128)
Accession # Q9QXU7
C-term
Protein Length

Full Length of Mature Protein
Synonyms
Prokineticin-2; Prok2; Protein Bv8 homolog; Bv8
AA Sequence

AVITGACDKDSQCGGGMCCAVSIWVKSIRICTPMGQVGDSCHPLTRKSHVANGRQERRRAKRRKRKKEVPFWGRRMHHTCPCLPGLACLRTSFNRFICLARK

Predicted Molecular Mass
29 kDa
Molecular Weight

Approximately 29 kDa,based on SDS-PAGE under reducing conditions.
Structure/Form
Monomer
Purity

Greater than 90% as determined by reducing SDS-PAGE.
Endotoxin Level

<1 EU/μg, determined by LAL method.
Documentation
Handling Instructions (2659 KB)

Prok2 Protein, Mouse (His-B2M) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • Reconstitution Calculator

  • Dilution Calculator

  • Specific Activity Calculator

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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Prok2 Protein, Mouse (His-B2M)
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