PNPLA3 Protein, Human (I148M, sf9, His)

PNPLA3 Protein, Human (I148M, sf9, His) is the recombinant human-derived PNPLA3 protein, expressed by Sf9 insect cells, with C-6*His tag.

PNPLA3 specifically catalyzes the coenzyme A (CoA)-dependent acylation of 1-acyl-sn-glycerol 3-phosphate (2-lysophosphatidic acid/LPA) to produce phosphatidic acid (PA), a key metabolic intermediate and precursor for both triglycerides and glycerophospholipids. It does not esterify other lysophospholipids, utilizing long-chain (at least C16) fatty acyl-CoAs such as arachidonoyl-CoA, linoleoyl-CoA, oleoyl-CoA, and to a lesser extent, palmitoyl-CoA as acyl donors. Additionally, PNPLA3 exhibits low triacylglycerol lipase and CoA-independent acylglycerol transacylase activities, suggesting a potential role in triglyceride acyl-chain remodeling. In vitro studies indicate hydrolytic activity against triacylglycerol, diacylglycerol, and monoacylglycerol, with a strong preference for oleic acid as the acyl moiety. However, its triacylglycerol hydrolase activity is debated and may be minimal. PNPLA3 also possesses phospholipase A2 activity. by similar: The enzyme's activity varies across studies, particularly regarding the extent of its triacylglycerol hydrolase function.

Human

Sf9 insect cells

C-6*His

Q9NST1 (M1-L481, I148M)

80339

Full Length

MYDAERGWSLSFAGCGFLGFYHVGATRCLSEHAPHLLRDARMLFGASAGALHCVGVLSGIPLEQTLQVLSDLVRKARSRNIGIFHPSFNLSKFLRQGLCKCLPANVHQLISGKIGISLTRVSDGENVLVSDFRSKDEVVDALVCSCFMPFYSGLIPPSFRGVRYVDGGVSDNVPFIDAKTTITVSPFYGEYDICPKVKSTNFLHVDITKLSLRLCTGNLYLLSRAFVPPDLKVLGEICLRGYLDAFRFLEEKGICNRPQPGLKSSSEGMDPEVAMPSWANMSLDSSPESAALAVRLEGDELLDHLRLSILPWDESILDTLSPRLATALSEEMKDKGGYMSKICNLLPIRIMSYVMLPCTLPVESAIAIVQRLVTWLPDMPDDVLWLQWVTSQVFTRVLMCLLPASRSQMPVSSQQASPCTPEQDWPCWTPCSPKGCPAETKAEATPRSILRSSLNFFLGNKVPAGAEGLSTFPSFSLEKSL

Approximately 56.0 kDa

Greater than 85% as determined by reducing SDS-PAGE.

Lyophilized powder.

Lyophilized from a 0.22 μm filtered solution of PBS, 6% trehalose, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.