PLK4 Protein, Human

PLK4 Protein, Human is the recombinant human-derived PLK4, expressed by E. coli , with tag Free labeled tag. ,

PLK4 is a serine/threonine-protein kinase that plays a central role in centriole duplication by triggering procentriole formation on parental centrioles and recruiting biogenesis proteins like SASS6, CPAP, and gamma-tubulin. Overexpression induces centrosome amplification via multiple procentriole generation during S phase. It phosphorylates FBXW5 at Ser-151 to suppress SASS6 ubiquitination and regulates G1/S transition. Dysregulation may contribute to tumorigenesis due to frequent centrosome aberrations in tumors. PLK4 also mediates deuterosome-dependent centriole amplification (producing >100 centrioles) in multiciliated cells and promotes trophoblast differentiation by phosphorylating HAND1. Additional substrates include CDC25C, CHEK2, CEP131 (Ser-78), and PCM1 (Ser-372), the latter being critical for centriolar satellite integrity. by similar: PLK4 is required for STIL recruitment and STIL-driven centriole amplification.

Human

E. coli

Tag Free

O00444-1 (A2-D275)

10733

Partial

31.3 KDa

Greater than 85% as determined by reducing SDS-PAGE

Solution

Supplied as a 0.22 μm filtered solution of 50mM Tris-HCl (pH 7.5), 250mM NaCl.

<1 EU/μg, determined by LAL method.

Please use rapid thawing with running water to thaw the protein.

Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping with dry ice.