2. Recombinant Proteins
  3. Enzymes & Regulators
  4. PIK3R2 Protein, Human (sf9, His)

PIK3R2 Protein, Human (sf9, His) is the recombinant human-derived PIK3R2, expressed by Sf9 insect cells , with His labeled tag. ,

For research use only. We do not sell to patients.

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Description

PIK3R2 Protein, Human (sf9, His) is the recombinant human-derived PIK3R2, expressed by Sf9 insect cells , with His labeled tag. ,
Background

PI3 Kinase p85 β is a regulatory subunit of phosphoinositide-3-kinase (PI3K), which phosphorylates PtdIns(4,5)P2 (Phosphatidylinositol 4,5-bisphosphate) to generate phosphatidylinositol 3,4,5-trisphosphate (PIP3). PIP3 recruits PH domain-containing proteins, such as AKT1 and PDPK1, to the membrane, activating signaling cascades involved in cell growth, survival, proliferation, motility, and morphology. p85 β binds to activated (phosphorylated) protein-tyrosine kinases via its SH2 domain, acting as an adapter to mediate the association of the p110 catalytic unit with the plasma membrane. It also indirectly regulates autophagy and promotes nuclear translocation of XBP1 isoform 2 in an ER stress- and/or insulin-dependent manner during metabolic overloading, thereby improving glucose tolerance (By similarity).
Species

Human
Source

Sf9 insect cells
Tag

His
Accession

O00459 (M1-R728)
Gene ID

5296
Protein Length

Full Length
Synonyms
PI3K (p85 beta); Phosphatidylinositol 3-kinase regulatory subunit beta; PI3-kinase regulatory subunit beta; PI3K regulatory subunit beta; PtdIns-3-kinase regulatory subunit beta; Phosphatidylinositol 3-kinase 85 kDa regulatory subunit beta (PI3-kinase subunit p85-beta; PtdIns-3-kinase regulatory subunit p85-beta)
Predicted Molecular Mass
88 kDa
Purity

Greater than 85% as determined by reducing SDS-PAGE
Documentation
Handling Instructions (2659 KB)

PIK3R2 Protein, Human (sf9, His) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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