2. Recombinant Proteins
  3. Enzymes & Regulators
  4. Hydrolases (EC 3)
  5. Phospholipase C Protein, Bacillus cereus

Phospholipase C Protein, Bacillus cereus is the recombinant Phospholipase C protein, expressed by E. coli, with tag free.

For research use only. We do not sell to patients.

Surface Plasmon Resonance (SPR) Assay Service   Protein Expression Service

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Top Publications Citing Use of Products

  • Biological Activity

  • Technical Parameters

  • Properties

  • Documentation

Description

Phospholipase C Protein, Bacillus cereus is the recombinant Phospholipase C protein, expressed by E. coli, with tag free.
Background

Phospholipase C is an enzyme that, in conjunction with sphingomyelinase, facilitates target cell lysis (hemolysis). by similar: requires cooperation with sphingomyelinase.
Species

Others
Source

E. coli
Tag

Tag Free
Accession

Q81HW1 (M1-R283)
Gene ID

72447479
Molecular Construction
N-term
Phospholipase C (M1-R283)
Accession # Q81HW1
C-term
Protein Length

Full Length
Synonyms
BC_0670; Phosphatidylcholine cholinephosphohydrolase; Phospholipase C
Molecular Weight

30.7 kDa
Purity

Greater than 90% as determined by reducing SDS-PAGE.
Appearance

Solution.
Formulation

Supplied as a 0.22 μm filtered solution of 50 mM Tris-HCl, pH7.5, 200 mM NaCl, 20% glycerol.
Endotoxin Level

<1 EU/μg, determined by LAL method.
Reconstitution

Please use rapid thawing with running water to thaw the protein.
Storage & Stability

Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping

Shipping with dry ice.
Documentation
SDS (251 KB)

COA (219 KB)

Handling Instructions (2659 KB)

Phospholipase C Protein, Bacillus cereus Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • Reconstitution Calculator

  • Dilution Calculator

  • Specific Activity Calculator

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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Phospholipase C Protein, Bacillus cereus
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