2. Recombinant Proteins
  3. Enzymes & Regulators
  4. Hydrolases (EC 3)
  5. PHLPP2 Protein, Human (GST)

PHLPP2 Protein, Human (GST) is the recombinant human-derived PHLPP2, expressed by E. coli , with GST labeled tag. ,

For research use only. We do not sell to patients.

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Description

PHLPP2 Protein, Human (GST) is the recombinant human-derived PHLPP2, expressed by E. coli , with GST labeled tag. ,
Background

PHLPP2 is a protein phosphatase involved in the regulation of Akt and PKC signaling pathways. It mediates dephosphorylation of the C-terminal hydrophobic motif in members of the AGC Ser/Thr protein kinase family, specifically targeting 'Ser-473' of AKT1, 'Ser-660' of PRKCB isoform beta-II, and 'Ser-657' of PRKCA. Akt regulates the balance between cell survival and apoptosis by modulating transcription factors that control pro- and anti-apoptotic genes. Dephosphorylation of Akt at 'Ser-473' by PHLPP2 promotes apoptosis and reduces cell proliferation. PHLPP2 also regulates the phosphorylation of AKT3 and dephosphorylates STK4 at 'Thr-387', leading to STK4 activation and apoptosis. Additionally, it dephosphorylates RPS6KB1, influencing cap-dependent translation. PHLPP2 inhibits cancer cell proliferation and may function as a tumor suppressor. Dephosphorylation of PRKCA and PRKCB by PHLPP2 destabilizes and degrades these proteins. Furthermore, PHLPP2 dephosphorylates RAF1, suppressing its kinase activity.
Species

Human
Source

E. coli
Tag

GST
Accession

Q6ZVD8-1 (K766-E1043)
Gene ID

23035
Protein Length

Partial
Synonyms
KIAA0931; PHLPPL
Predicted Molecular Mass
56 kDa
Purity

Greater than 95% as determined by reducing SDS-PAGE
Documentation
Handling Instructions (2659 KB)

PHLPP2 Protein, Human (GST) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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