PAK6 Protein, Human

PAK6, a dynamic serine/threonine kinase, regulates gene transcription activated by AR or MAP2K6/MAPKK6. It phosphorylates and inhibits AR-mediated transcription, hindering ESR1-mediated transcription. PAK6 interacts with IQGAP1, suggesting a role in cytoskeleton regulation. Moreover, it protects cells from apoptosis by phosphorylating BAD, showcasing its multifaceted impact on transcriptional control and cytoskeletal dynamics. PAK6 Protein, Human is the recombinant human-derived PAK6 protein, expressed by E. coli , with tag free.

PAK6, a serine/threonine protein kinase, serves as a regulator of gene transcription, with its kinase activity triggered by various effectors, including AR or MAP2K6/MAPKK6. Notably, it phosphorylates the DNA-binding domain of the androgen receptor/AR, hindering AR-mediated transcription, and also inhibits ESR1-mediated transcription. PAK6's involvement in cytoskeleton regulation is suggested by its interaction with IQGAP1. Additionally, PAK6 demonstrates a potential role in cell survival by protecting cells from apoptosis through the phosphorylation of BAD. This multifaceted kinase exhibits a regulatory impact on key cellular processes, showcasing its significance in transcriptional control and cytoskeletal dynamics.

Human

E. coli

Tag Free

Q9NQU5 (G383-Y674)

106821730

Partial

Approximately 33.2 KDa.

Greater than 90% as determined by reducing SDS-PAGE.

Solution.

Supplied as a 0.22 μm filtered solution of 50 mM Tris-HCl, pH 7.5, 500 mM NaCl, 10% glycerol, 1 mM DTT.
Note: For SPR assay, please replace the buffer. Primary amine components (e.g., Tris, imidazole) can affect protein-coupled chips.

<1 EU/μg, determined by LAL method.

Please use rapid thawing with running water to thaw the protein.

Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping with dry ice.