1. Recombinant Proteins
  2. Enzymes & Regulators
  3. Hydrolases (EC 3)
  4. PAD1 Protein, Human (sf9, His)

PAD1 Protein, Human (sf9, His) is the recombinant human-derived PAD1 protein, expressed by sf9 insect cells, with N-His tag.

For research use only. We do not sell to patients.

Surface Plasmon Resonance (SPR) Assay Service   Protein Expression Service

Size Price Stock Quantity
50 μg Get quote 3 - 4 weeks 2 - 3 weeks 2 - 3 weeks
100 μg   Get quote  
Synthetic products have potential research and development risk.

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Top Publications Citing Use of Products
  • Biological Activity

  • Technical Parameters

  • Properties

  • Documentation

Description

PAD1 Protein, Human (sf9, His) is the recombinant human-derived PAD1 protein, expressed by sf9 insect cells, with N-His tag.

Background

PAD1 is an enzyme that catalyzes the deimination of arginine residues in proteins. by similar: This function is analogous to other members of the peptidylarginine deiminase (PAD) family.

Species

Human

Source

sf9 insect cells

Tag

N-His

Accession

Q9ULC6 (M1-P663)

Gene ID

29943

Molecular Construction
N-term
His
PAD1(M1-P663)
Accession # Q9ULC6
C-term
Synonyms
PAD1; PDI1
Predicted Molecular Mass
76.7 kDa.
Molecular Weight

Approximately 47 kDa and 65-70 kDa, based on SDS-PAGE under reducing conditions.

Purity

Greater than 95% as determined by reducing SDS-PAGE.

Appearance

Solution.

Formulation

Supplied as 0.22 μm filtered solution of 50 mM Tris, 150 mM NaCl, pH7.5 with 50% glycerol as protectant.
Note: For SPR assay, please replace the buffer. Primary amine components (e.g., Tris, imidazole) can affect protein-coupled chips.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

N/A.

Storage & Stability

Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping

Shipping with dry ice.

Documentation

PAD1 Protein, Human (sf9, His) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • Reconstitution Calculator

  • Dilution Calculator

  • Specific Activity Calculator

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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Product Name:
PAD1 Protein, Human (sf9, His)
Cat. No.:
HY-P704834
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