P2Y1 Protein, Human (sf9, FLAG)

The P2Y1 protein acts as a receptor for extracellular adenine nucleotides, specifically ADP. In platelets, ADP binding activates phospholipase C, leading to intracellular calcium mobilization, platelet shape changes, and aggregation. P2Y1 Protein, Human (sf9, FLAG) is the recombinant human-derived P2Y1 protein, expressed by Sf9 insect cells, with C-10*His and N-Flag labeled tag.

The P2Y1 receptor serves as a receptor for extracellular adenine nucleotides, particularly ADP. Upon binding to ADP, this receptor, found notably in platelets, triggers the mobilization of intracellular calcium ions through the activation of phospholipase C. Consequently, this activation induces changes in platelet shape and, ultimately, platelet aggregation. ATP acts as an antagonist, inhibiting the ADP-induced mobilization of calcium ions. Specific antagonists of the P2Y1 receptor, such as A3P5PS, A3P5P, and A2P5P, have been identified and shown to impede downstream signaling events mediated by the mobilization of calcium ions from intracellular stores, as well as platelet shape changes in response to extracellular ADP.

Human

Sf9 insect cells

C-10*His;N-Flag

P47900 (T2-K247&M1-E54 of Rubredoxin&P253-L373, D320N)

5028

Partial

DYKDDDDKTEVLWPAVPNGTDAAFLAGPGSSWGNSTVASTAAVSSSFKCALTKTGFQFYYLPAVYILVFIIGFLGNSVAIWMFVFHMKPWSGISVYMFNLALADFLYVLTLPALIFYYFNKTDWIFGDAMCKLQRFIFHVNLYGSILFLTCISAHRYSGVVYPLKSLGRLKKKNAICISVLVWLIVVVAISPILFYSGTGVRKNKTITCYDTTSDEYLRSYFIYSMCTTVAMFCVPLVLILGCYGLIVRALIYKMKKYTCTVCGYIYNPEDGDPDNGVNPGTDFKDIPDDWVCPLCGVGKDQFEEVEEPLRRKSIYLVIIVLTVFAVSYIPFHVMKTMNLRARLDFQTPAMCAFNDRVYATYQVTRGLASLNSCVNPILYFLAGDTFRRRLSRATRKASRRSEANLQSKSEDMTLNILPEFKQNGDTSLLEVLFQGPHHHHHHHHHH

50.7 kDa

Greater than 90% as determined by reducing SDS-PAGE.

Solution

Supplied as a 0.2 μm filtered solution of 20 mM HEPES pH 7.5, 150 mM NaCl, 0.01%, w/v LMNG, 0.001%, w/v CHS.

<1 EU/μg, determined by LAL method.

Please use rapid thawing with running water to thaw the protein.

Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping with dry ice.