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  3. P2X3 Protein, Human (HEK293)

P2X3 Protein, Human (HEK293) is the recombinant human-derived P2X3, expressed by HEK293 , with tag Free labeled tag. ,

For research use only. We do not sell to patients.

Surface Plasmon Resonance (SPR) Assay Service   Protein Expression Service

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  • Biological Activity

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Description

P2X3 Protein, Human (HEK293) is the recombinant human-derived P2X3, expressed by HEK293 , with tag Free labeled tag. ,

Background

P2X3 is an extracellular ATP-activated non-selective cation channel (PubMed:10440098, PubMed:27626375, PubMed:29674445, PubMed:31232692). It plays a particularly important role in sensory neurons, where its activation is critical for gustatory, nociceptive responses, visceral reflexes, and sensory hypersensitization (By similarity).

Species

Human

Source

HEK293

Tag

Tag Free

Accession

P56373 (D6-E363, T13P, S15V, V16I)

Gene ID

5024

Protein Length

Partial

Synonyms
P2X purinoceptor 3; ATP receptor; Purinergic receptor; P2RX3; Homo sapiens; Human; P2X3; Ion channel; Ligand-gated ion channel; Receptor
Molecular Weight

Approximately 40.4 kDa

Purity

Greater than 90% as determined by reducing SDS-PAGE

Appearance

Solution

Formulation

Supplied as a 0.22 μm filtered solution of 20 mM HEPES pH 7.5, 150 mM NaCl, 0.03% (w/v) GDN, 0.5 mM TCEP.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

Please use rapid thawing with running water to thaw the protein.

Storage & Stability

Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping

Shipping with dry ice.

Documentation

P2X3 Protein, Human (HEK293) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • Reconstitution Calculator

  • Dilution Calculator

  • Specific Activity Calculator

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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Product Name:
P2X3 Protein, Human (HEK293)
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HY-P703172
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