Osteopontin/OPN Protein, Human (HEK293, His)

Osteopontin/OPN Protein, a key non-collagenous bone protein, strongly binds to hydroxyapatite, contributing to the mineralized matrix. Its significant role in cell-matrix interactions is evident. Acting as a cytokine, OPN promotes interferon-gamma and interleukin-12 production while suppressing interleukin-10, crucial for type I immunity. Osteopontin/OPN Protein, Human (HEK293, His) is the recombinant human-derived Osteopontin/OPN protein, expressed by HEK293 , with C-6*His labeled tag.

Osteopontin (OPN) protein is a significant non-collagenous bone protein that exhibits a strong binding affinity to hydroxyapatite, making it an integral component of the mineralized matrix. It likely plays a crucial role in cell-matrix interactions. Moreover, OPN acts as a cytokine, facilitating the production of interferon-gamma and interleukin-12 while suppressing the production of interleukin-10. These functions are vital in the pathway that promotes type I immunity.

Measured by the ability of the immobilized protein to support the adhesion of HEK293 human embryonic kidney cells. When 1 x 10^5 cells/well are added to Recombinant Human Osteopontin coated plates, cell adhesion is enhanced in a dose dependent manner after 1 hour incubation at 37 °C. The ED₅₀ for this effect is <0.3 μg/mL.

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  Measured by the ability of the immobilized protein to support the adhesion of HEK293 human embryonic kidney cells. When 1 x 10⁵ cells/well are added to Recombinant Human Osteopontin coated plates, cell adhesion is enhanced in a dose dependent manner after 1 hour incubation at 37 °C. The ED₅₀ for this effect is 0.1486 μg/mL, corresponding to a specific activity is 6729.475 units/mg.

Human

HEK293

C-6*His

P10451-1 (I17-N314)

6696  [NCBI]

IPVKQADSGSSEEKQLYNKYPDAVATWLNPDPSQKQNLLAPQNAVSSEETNDFKQETLPSKSNESHDHMDDMDDEDDDDHVDSQDSIDSNDSDDVDDTDDSHQSDESHHSDESDELVTDFPTDLPATEVFTPVVPTVDTYDGRGDSVVYGLRSKSKKFRRPDIQYPDATDEDITSHMESEELNGAYKAIPVAQDLNAPSDWDSRGKDSYETSQLDDQSAETHSHKQSRLYKRKANDESNEHSDVIDSQELSKVSREFHSHEFHSHEDMLVVDPKSKEEDKHLKFRISHELDSASSEVN

Approximately 50-69 kDa due to the glycosylation

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  Greater than 95% as determined by reducing SDS-PAGE.

Lyophilized powder

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4 or PBS, pH 7.4, 8% trehalose.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.