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  4. OLFML3 Protein, Human (HEK293, His)

OLFML3 Protein, Human (HEK293, His) is the recombinant human-derived OLFML3 protein, expressed by HEK293, with C-His tag.

For research use only. We do not sell to patients.

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Description

OLFML3 Protein, Human (HEK293, His) is the recombinant human-derived OLFML3 protein, expressed by HEK293, with C-His tag.
Background

OLFML3 is a secreted scaffold protein essential for dorsoventral patterning during early development. It stabilizes axial formation by restricting chordin (CHRD) activity on the dorsal side, achieved through facilitating the association between tolloid proteases and their substrate chordin (CHRD), thereby enhancing chordin (CHRD) degradation (By similarity). It may also have matrix-related functions involved in placental and embryonic development or play similar roles in other physiological processes.
Species

Human
Source

HEK293
Tag

C-His
Accession

Q9NRN5-1 (K120-V406)
Gene ID

56944
Molecular Construction
N-term
OLFML3 (K120-V406)
Accession # Q9NRN5-1
His
C-term
Protein Length

Partial
Synonyms
Olfactomedin-like protein 3,HNOEL-iso,Holf44,OLFML3,PSEC0035, PSEC0173, PSEC0244, UNQ663,PRO1294
AA Sequence

KGKGRRNEKYDMVTDCGYTISQVRSMKILKRFGGPAGLWTKDPLGQTEKIYVLDGTQNDTAFVFPRLRDFTLAMAARKASRVRVPFPWVGTGQLVYGGFLYFARRPPGRPGGGGEMENTLQLIKFHLANRTVVDSSVFPAEGLIPPYGLTADTYIDLAADEEGLWAVYATREDDRHLCLAKLDPQTLDTEQQWDTPCPRENAEAAFVICGTLYVVYNTRPASRARIQCSFDASGTLTPERAALPYFPRRYGAHASLRYNPRERQLYAWDDGYQIVYKLEMRKKEEEV

Predicted Molecular Mass
34.4 kDa
Molecular Weight

Approximately 38-45 kDa,based on SDS-PAGE under reducing conditions,due to the glycosylation.
Glycosylation
Yes
Structure/Form
Monomer
Purity

Greater than 95% as determined by reducing SDS-PAGE.
Endotoxin Level

<1 EU/μg, determined by LAL method.
Documentation
Handling Instructions (2659 KB)

OLFML3 Protein, Human (HEK293, His) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • Reconstitution Calculator

  • Dilution Calculator

  • Specific Activity Calculator

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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