Nucleocapsid Protein, Virus (His)

The SARS-CoV-2 nucleocapsid protein plays a crucial role in assembling the virion, forming the helical ribonucleocapsid (RNP), and interacting with the viral genome and membrane protein M.In addition to its structural function, it also enhances subgenomic viral RNA transcription and overall replication.Nucleocapsid Protein, Virus (His) is the recombinant Virus-derived Nucleocapsid protein, expressed by E.coli , with N-6*His labeled tag.

The SARS-CoV-2 Nucleocapsid Protein assumes a pivotal role in virion assembly, orchestrating the packaging of the positive-strand viral genome RNA into a helical ribonucleocapsid (RNP) and establishing crucial interactions with the viral genome and membrane protein M. Beyond its structural functions, this protein significantly contributes to the efficiency of subgenomic viral RNA transcription and overall viral replication. Notably, it exhibits an additional role in modulating host chemokine function, a mechanism that may favor viral replication and transmission. The Nucleocapsid Protein achieves this modulation by being secreted into the extracellular space, where it competes with host chemokines for binding to host glycosaminoglycans (GAG), thus potentially disrupting host defense mechanisms and supporting the viral life cycle.

Virus

E. coli

N-6*His

QHD43423.2 (M1-A419)

43740575

Full Length

MSDNGPQNQRNAPRITFGGPSDSTGSNQNGERSGARSKQRRPQGLPNNTASWFTALTQHGKEDLKFPRGQGVPINTNSSPDDQIGYYRRATRRIRGGDGKMKDLSPRWYFYYLGTGPEAGLPYGANKDGIIWVATEGALNTPKDHIGTRNPANNAAIVLQLPQGTTLPKGFYAEGSRGGSQASSRSSSRSRNSSRNSTPGSSRGTSPARMAGNGGDAALALLLLDRLNQLESKMSGKGQQQQGQTVTKKSAAEASKKPRQKRTATKAYNVTQAFGRRGPEQTQGNFGDQELIRQGTDYKHWPQIAQFAPSASAFFGMSRIGMEVTPSGTWLTYTGAIKLDDKDPNFKDQVILLNKHIDAYKTFPPTEPKKDKKKKADETQALPQRQKKQQTVTLLPAADLDDFSKQLQQSMSSADSTQA

Approximately 48-55 kDa

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  Greater than 95% as determined by reducing SDS-PAGE.

Lyophilized powder

Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4, 8% trehalose.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.