NTN1 Protein, Human (HEK293, His)

Netrin-1 protein is a key axon guidance regulator that guides commissural and peripheral motor axons in the central nervous system. Binding to DCC or UNC5 receptors determines axonal attraction or repulsion. NTN1 Protein, Human (HEK293, His) is the recombinant human-derived NTN1 protein, expressed by HEK293, with C-His labeled tag.

Netrin-1, a crucial regulator of axonal guidance in both the central nervous system (CNS) and peripheral motor neurons, plays a pivotal role in directing commissural axons within the CNS and motor axons in the periphery. The association of Netrin-1 with either DCC or certain UNC5 receptors dictates whether axons are attracted or repelled. Specifically, binding to UNC5C induces the dissociation of UNC5C from polymerized TUBB3 in microtubules, leading to heightened microtubule dynamics and subsequent axon repulsion. Netrin-1 is essential for dorsal root ganglion axon projection toward the spinal cord and functions as a survival factor by preventing the initiation of apoptosis through its interactions with receptors. Furthermore, Netrin-1's involvement in tumorigenesis underscores its regulatory role in apoptosis. The protein binds to a repertoire of receptors, including DCC, UNC5A, UNC5B, UNC5C, UNC5D, and DSCAM, and exhibits interaction with APP, highlighting its multifaceted roles in cellular processes.

Human

HEK293

C-His

O95631-1 (V22-A604)

9423

Full Length of Isoform-1 Mature Protein

VRGGPGLSMFAGQAAQPDPCSDENGHPRRCIPDFVNAAFGKDVRVSSTCGRPPARYCVVSERGEERLRSCHLCNASDPKKAHPPAFLTDLNNPHNLTCWQSENYLQFPHNVTLTLSLGKKFEVTYVSLQFCSPRPESMAIYKSMDYGRTWVPFQFYSTQCRKMYNRPHRAPITKQNEQEAVCTDSHTDMRPLSGGLIAFSTLDGRPSAHDFDNSPVLQDWVTATDIRVAFSRLHTFGDENEDDSELARDSYFYAVSDLQVGGRCKCNGHAARCVRDRDDSLVCDCRHNTAGPECDRCKPFHYDRPWQRATAREANECVACNCNLHARRCRFNMELYKLSGRKSGGVCLNCRHNTAGRHCHYCKEGYYRDMGKPITHRKACKACDCHPVGAAGKTCNQTTGQCPCKDGVTGITCNRCAKGYQQSRSPIAPCIKIPVAPPTTAASSVEEPEDCDSYCKASKGKLKINMKKYCKKDYAVQIHILKADKAGDWWKFTVNIISVYKQGTSRIRRGDQSLWIRSRDIACKCPKIKPLKKYLLLGNAEDSPDQSGIVADKSSLVIQWRDTWARRLRKFQQREKKGKCKKA

Approximately 80-100 kDa, based on SDS-PAGE under reducing conditions, due to the glycosylation.

Greater than 95% as determined by reducing SDS-PAGE.

Lyophilized powder.

Lyophilized from a 0.22 μm filtered solution of PBS, pH7.4 with NaCl with trehalose as protectant.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.