NR5A1 Protein, Human (His, Strep)

NR5A1 Protein, Human (His, Strep) is the recombinant human-derived NR5A1, expressed by E. coli , with Strep, His labeled tag. ,

NR5A1 is a transcriptional activator essential for sexual differentiation and the formation of primary steroidogenic tissues. It binds to the Ad4 site in the promoter regions of steroidogenic P450 genes such as CYP11A, CYP11B, and CYP21B, and regulates genes like AMH/Muellerian inhibiting substance, ACH, and STAR. Its recognition consensus sequences are 5'-YCAAGGYC-3' and 5'-RRAGGTCA-3'. The SFPQ-NONO-NR5A1 complex binds to the CYP17 promoter, regulating basal and cAMP-dependent transcriptional activity. NR5A1 also binds phosphatidylcholine (by similar) and phospholipids with a phosphatidylinositol (PI) headgroup, particularly PI(3,4)P2 and PI(3,4,5)P3. Phosphorylation of NR5A1 by HIPK3 activates it, enhancing steroidogenic gene expression upon cAMP signaling pathway stimulation.

Human

E. coli

Strep;His

Q13285 (S218-T461, C247S, C412S)

2516

Partial

Greater than 95% as determined by reducing SDS-PAGE

Solution.

Supplied as a 0.22 μm filtered solution of 50 mM HEPES (pH 7.5), 200mM NaCl, 20% glycerol, 1mM DTT.

<1 EU/μg, determined by LAL method.

Please use rapid thawing with running water to thaw the protein.

Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping with dry ice.