NOD2 Protein, Human (sf9, Strep, Flag)

NOD2 Protein, Human (sf9, Strep, Flag) is the recombinant human-derived NOD2, expressed by sf9 insect cells , with Strep, Flag labeled tag. ,

NOD2 is a pattern recognition receptor (PRR) that detects bacterial peptidoglycan fragments and danger signals, playing a key role in gastrointestinal immunity. It is specifically activated by muramyl dipeptide (MDP), a universal bacterial peptidoglycan component, and binds its phosphorylated form, 6-O-phospho-MDP. This binding triggers oligomerization, recruiting and activating RIPK2, which undergoes ubiquitination by E3 ligases (XIAP, BIRC2, BIRC3, and LUBAC), leading to NF-kappa-B and MAP kinase signaling activation and immune gene transcription. NOD2 maintains gut microbiota-host immune balance via MDP sensing (by similar). Dysregulation causes dysbiosis, epithelial barrier damage, and inflammation (by similar). It also regulates appetite by sensing MDP in brain neurons, suppressing neuronal activity to modulate satiety and body temperature (by similar). Under malnutrition, intestinal MDP sensing supports postnatal growth (by similar). NOD2 senses viral ssRNA, interacting with MAVS to activate IRF3 and type I interferon. It modulates dendritic cell autophagy via ATG16L1 and promotes intestinal stem cell survival (by similar). Additionally, NOD2 responds to endoplasmic reticulum stress by detecting sphingosine-1-phosphate, initiating inflammatory signaling, and may activate NLRP1 in macrophages, leading to CASP1 activation and IL1B release.

Human

Sf9 insect cells

N-Flag;N-StrepⅡ

Q9HC29-1 (P213-L1040, delCARD)

64127

Partial

Approximately 94.5 kDa

Greater than 90% as determined by reducing SDS-PAGE

Solution

Supplied as a 0.22 μm filtered solution of 25 mM Tris-HCl, pH 8.0, 500 mM NaCl, 1 mM DTT, 10% glycerol, 5 mM MgCl2.
Note: For SPR assay, please replace the buffer. Primary amine components (e.g., Tris, imidazole) can affect protein-coupled chips.

<1 EU/μg, determined by LAL method.

Please use rapid thawing with running water to thaw the protein.

Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping with dry ice.