Neuropilin-1 Protein, Human (HEK293, mFc)

Neuropilin-1 protein plays an important role by binding to VEGF-165, possibly inhibiting its cellular binding and inducing apoptosis. This dual function suggests its role in regulating VEGF-mediated processes. Neuropilin-1 Protein, Human (HEK293, mFc) is the recombinant human-derived Neuropilin-1 protein, expressed by HEK293 , with C-mFc labeled tag. The total length of Neuropilin-1 Protein, Human (HEK293, mFc) is 623 a.a., with molecular weight of 110-130 kDa.

The Neuropilin-1 protein assumes a significant role as it binds to VEGF-165, potentially inhibiting its binding to cells and inducing apoptosis by sequestering VEGF-165. This dual functionality suggests Neuropilin-1's involvement in the regulation of VEGF-mediated cellular processes. Additionally, Neuropilin-1 may interact with various members of the semaphorin family, indicating its versatility in binding to different ligands. Notably, its expression appears to exert an adverse effect on blood vessel number and integrity, suggesting a potential role in angiogenesis and vascular development. The diverse interactions and regulatory effects of Neuropilin-1 underscore its importance in orchestrating complex cellular processes and its potential implications in angiogenesis and apoptotic pathways.

Immobilized Human VEGF165 His at 2 μg/mL (100 μL/well) can bind Human Neuropilin-1 mFc with a linear range of 0.2-5 ng/mL.

Human

HEK293

C-mFc

AAH07533 (F22-K644)

8829  [NCBI]

110-130 kDa

Greater than 95% as determined by reducing SDS-PAGE.

Lyophilized powder.

Lyophilized a 0.22 μm filtered solution of 50 mM Tris, 100 mM Glycine, 25 mM Arginine, 150 mM NaCl, pH 7.5. Normally trehalose is added as protectant before lyophilization.
Note: For SPR assay, please replace the buffer. Primary amine components (e.g., Tris, imidazole) can affect protein-coupled chips.

<0.1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.