Neurofascin Protein, Human (HEK293, C-His)

Neurofascin Protein, Human (HEK293, C-His) is the recombinant human-derived Neurofascin, expressed by HEK293 , with C-10*His labeled tag. ,

NFASC, a cell adhesion and ankyrin-binding protein, is implicated in various neural processes, including neurite extension, axonal guidance, synaptogenesis, myelination, and interactions between neurons and glial cells. It forms a horseshoe-shaped homodimer and is likely a component of the NFASC/NRCAM/ankyrin-G complex. NFASC associates with sodium channel beta-1 (SCN1B) and beta-3 (SCN3B) subunits, indicating its involvement in neuronal excitability and ion channel regulation. Additionally, NFASC interacts with GLDN/gliomedin, emphasizing its role in nodes of Ranvier and myelination. Furthermore, it engages with MYOC, suggesting potential connections to ocular functions or glaucoma. The multifaceted interactions of NFASC underscore its significance in orchestrating diverse cellular processes within the nervous system.

Measured in a cell proliferation assay using SH-SY5Y cells. The ED₅₀ for this effect is 1.498 μg/mL, corresponding to a specific activity is 667.557 units/mg.

Human

HEK293

C-10*His

O94856-12 (I25-Q939)

23114

Partial

IEIPMDPSIQNELTQPPTITKQSAKDHIVDPRDNILIECEAKGNPAPSFHWTRNSRFFNIAKDPRVSMRRRSGTLVIDFRSGGRPEEYEGEYQCFARNKFGTALSNRIRLQVSKSPLWPKENLDPVVVQEGAPLTLQCNPPPGLPSPVIFWMSSSMEPITQDKRVSQGHNGDLYFSNVMLQDMQTDYSCNARFHFTHTIQQKNPFTLKVLTTRGVAERTPSFMYPQGTASSQMVLRGMDLLLECIASGVPTPDIAWYKKGGDLPSDKAKFENFNKALRITNVSEEDSGEYFCLASNKMGSIRHTISVRVKAAPYWLDEPKNLILAPGEDGRLVCRANGNPKPTVQWMVNGEPLQSAPPNPNREVAGDTIIFRDTQISSRAVYQCNTSNEHGYLLANAFVSVLDVPPRMLSPRNQLIRVILYNRTRLDCPFFGSPIPTLRWFKNGQGSNLDGGNYHVYENGSLEIKMIRKEDQGIYTCVATNILGKAENQVRLEVKDPTRIYRMPEDQVARRGTTVQLECRVKHDPSLKLTVSWLKDDEPLYIGNRMKKEDDSLTIFGVAERDQGSYTCVASTELDQDLAKAYLTVLADQATPTNRLAALPKGRPDRPRDLELTDLAERSVRLTWIPGDANNSPITDYVVQFEEDQFQPGVWHDHSKYPGSVNSAVLRLSPYVNYQFRVIAINEVGSSHPSLPSERYRTSGAPPESNPGDVKGEGTRKNNMEITWTPMNATSAFGPNLRYIVKWRRRETREAWNNVTVWGSRYVVGQTPVYVPYEIRVQAENDFGKGPEPESVIGYSGEDLPSAPRRFRVRQPNLETINLEWDHPEHPNGIMIGYTLKYVAFNGTKVGKQIVENFSPNQTKFTVQRTDPVSRYRFTLSARTQVGSGEAVTEESPAPPNEATPTAAYTNNQADIATQ

Approximately 116 kDa

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  Greater than 95% as determined by reducing SDS-PAGE.

Lyophilized powder.

Lyophilized from a 0.22 μm filtered solution of 20 mM Tris-HCl, 150 mM NaCl, pH 8.5.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.