Nectin-3/CD113 Protein, Human (Biotinylated, HEK293, Fc-Avi)

Nectin-3/CD113 is a multifunctional cell adhesion protein that mediates cell-cell interactions through heterophilic trans binding to nectin-like proteins or nectins. It interacts with NECTIN2 at the Sertoli cell-spermatocyte junction and triggers CDC42 and RAC small G protein activation through common signaling molecules such as SRC and RAP1. Nectin-3/CD113 Protein, Human (Biotinylated, HEK293, Fc-Avi) is the recombinant human-derived Nectin-3/CD113 protein, expressed by HEK293 , with C-Avi, C-hFc labeled tag.

Nectin-3/CD113, a versatile cell adhesion protein, plays a pivotal role in mediating cell-cell interactions through heterophilic trans-binding with nectin-like proteins or nectins, exemplified by its interaction with NECTIN2 at Sertoli-spermatid junctions. This trans-interaction with PVR triggers the activation of CDC42 and RAC small G proteins, mediated by common signaling molecules such as SRC and RAP1. Beyond its involvement in adherens junctions and synapses, Nectin-3/CD113 induces endocytosis-mediated down-regulation of PVR from the cell surface, leading to a reduction in cell movement and proliferation. Furthermore, it contributes to the morphology of the ciliary body and forms cis- and trans-homodimers, as well as trans-heterodimers with various proteins, including NECTIN1, NECTIN2, PVR, IGSF4B/Necl-1, and IGSF4. The interaction between NECTIN1 and NECTIN3 at pre- and postsynaptic sites initiates the formation of puncta adherentia junctions between axons and dendrites. Additionally, Nectin-3/CD113 binds with low affinity to TIGIT and interacts with AFDN, establishing a connection with the actin cytoskeleton.

Immobilized Human Nectin-1 His at 2 μg/mL (100 μL/well) can bind Biotinylated Human Nectin-3 Fc-Avi with a linear range of 0.078-0.625 μg/mL.

Human

HEK293

C-Avi;C-hFc

Q9NQS3-1 (G58-D400)

25945  [NCBI]

80-110 kDa

Greater than 95% as determined by reducing SDS-PAGE.

Lyophilized powder.

Lyophilized a 0.22 μm filtered solution of Tris with Glycine, Arginine and NaCl, pH 7.5. Normally trehalose is added as protectant before lyophilization.
Note: For SPR assay, please replace the buffer. Primary amine components (e.g., Tris, imidazole) can affect protein-coupled chips.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.