2. Recombinant Proteins
  3. Viral Proteins
  4. Influenza Viruses Proteins
  5. Influenza Virus Neuraminidase
  6. NA/Neuraminidase Protein, Influenza A H1N1 (HEK293)

NA/Neuraminidase Protein, Influenza A H1N1 (HEK293)

Cat. No.: HY-P73231
COA
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NA/Neuraminidase Protein is a receptor-destroying enzyme that cleaves terminal sialic acids from cellular receptors, potentially facilitating viral invasion of the upper airways by targeting sialic acid moieties on airway epithelial cell mucin. NA plays a pivotal role in viral propagation by catalyzing the removal of terminal sialic acid residues from both viral and cellular glycoconjugates. Moreover, the sialidase activity in late endosome/lysosome traffic appears to enhance virus replication. This product is an active extract, not a recombinant NA protein. It originates from cell lysates collected after expressing a DNA sequence encoding the Influenza A virus neuraminidase.

For research use only. We do not sell to patients.

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Description

NA/Neuraminidase Protein is a receptor-destroying enzyme that cleaves terminal sialic acids from cellular receptors, potentially facilitating viral invasion of the upper airways by targeting sialic acid moieties on airway epithelial cell mucin. NA plays a pivotal role in viral propagation by catalyzing the removal of terminal sialic acid residues from both viral and cellular glycoconjugates. Moreover, the sialidase activity in late endosome/lysosome traffic appears to enhance virus replication. This product is an active extract, not a recombinant NA protein. It originates from cell lysates collected after expressing a DNA sequence encoding the Influenza A virus neuraminidase.
Background

Neuraminidase (NA) plays a pivotal role in viral propagation by catalyzing the removal of terminal sialic acid residues from both viral and cellular glycoconjugates. Specifically, during virus budding, NA cleaves off terminal sialic acids from the glycosylated hemagglutinin (HA), facilitating the release of viral particles and enabling efficient virus spread through the circulation. By preventing self-aggregation and ensuring the removal of sialic acids from the cell surface, NA allows the progeny virus to disseminate efficiently from cell to cell, thereby avoiding limitations to a single round of replication. Described as a receptor-destroying enzyme, NA cleaves terminal sialic acids from cellular receptors, potentially facilitating viral invasion of the upper airways by targeting sialic acid moieties on airway epithelial cell mucin. Its association with lipid rafts during intracellular transport, and its potential raft-independent effect on budding, highlight the multifaceted role of NA in determining host range restriction, replication, and virulence. Moreover, the sialidase activity in late endosome/lysosome traffic appears to enhance virus replication[1][2][3].
Biological Activity

Measured by its ability to cleave a fluorogenic substrate , 2’-(4- Methylumbelliferyl)-α-D-N-acetylneuraminic acid. One unit is defined as the amount of enzyme required to cleave 1 nmole of 2'-(4-Methylumbelliferyl)-α-D-N-acetylneuraminic acid per minute at pH 7.5 at 37℃.
Species

Virus
Source

HEK293
Tag

Tag Free
Accession

ACP41107.1 (H36-K469)
Gene ID

/
Molecular Construction
N-term
H1N1 NA (H36-K469)
Accession # ACP41107.1
C-term
Protein Length

Partial
Synonyms
NA; Neuraminidase
Predicted Molecular Mass
47.8 kDa
Purity

\
Appearance

Lyophilized powder
Formulation

Lyophilized from a 0.2 μm filtered solution of PBS, 0.6% Triton X-100, 7% Trehalose, 6% Mannitol, pH 7.4.
Endotoxin Level

\
Reconstitution

It is recommended that 1 ml sterile water be added to the vial to prepare a stock solution.
Storage & Stability

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
Shipping

Room temperature in continental US; may vary elsewhere.
Documentation
SDS (251 KB)

COA (220 KB)

Handling Instructions (2659 KB)

NA/Neuraminidase Protein, Influenza A H1N1 (HEK293) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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  • Dilution Calculator

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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NA/Neuraminidase Protein, Influenza A H1N1 (HEK293)
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