Mucin-15/MUC15 Protein, Cynomolgus (HEK293, Fc)

MUC15 is a membrane-bound mucin. The human MUC15 protein contains an extracelluar domain, a small transmembrane domain, and a highly conserved cytoplasmic tail. MUC15 overexpression suppresses trophoblast-like cell invasion^[1][2]. Mucin-15/MUC15 Protein, Cynomolgus (HEK293, Fc) is the recombinant cynomolgus-derived Mucin-15/MUC15 protein, expressed by HEK293 , with C-hFc labeled tag.

MUC15 (mucin) was characterized because of its association with the bovine milk fat globule membrane (MFGM). It was shown to be a 33.3-kDa (307 AA) protein comprising an extracellular mucin domain rich in Ser, Thr, and Pro residues, a type 1 membrane-spanning domain, and a short cytoplasmic tail. In SDS-PAGE, bovine MUC15 migrates as a 115- to 130-kDa protein, which reflects its massive glycosylation (both N- and O-glycans). The corresponding cDNA of human MUC15 encodes an unprocessed protein of 334 AA showing 67% sequence similarity with the bovine counterpart^[1].
The membrane-bound mucins, which are type I membrane proteins, include MUC1, MUC3, MUC4, MUC12, MUC13, MUC15, MUC16, MUC17, and MUC20. Overexpression of MUC15 substantially decreased matrigel invasion of JAR and JEG-3 cells by 87.5 ± 1.1 and 83.8 ± 5.7%, respectively. The low levels of MUC15 expression may be critical for trophoblast invasion in early placental development, whereas high levels of MUC15 expression may provide a suppressive signal for trophoblast invasion at the end of gestation^[2].

Cynomolgus

HEK293

C-hFc

XP_005541632.1 (L254-G373)

102123068  [NCBI]

Approximately 48 kDa

Greater than 90% as determined by reducing SDS-PAGE.

<1 EU/μg, determined by LAL method.