MT-RNR1 Protein, Human (His, GST)

MT-RNR1 Protein, Human (His, GST) is the recombinant human-derived MT-RNR1, expressed by E. coli , with His, GST labeled tag. ,

MT-RNR1 regulates insulin sensitivity and metabolic homeostasis by inhibiting the folate cycle, reducing de novo purine biosynthesis, and accumulating the intermediate AICAR to activate AMPK. It protects against age-related and diet-induced insulin resistance and obesity. Under metabolic stress, it translocates to the nucleus, binds to AREs, and modulates gene expression in an NFE2L2-dependent manner to enhance cellular stress resistance. Additionally, it increases mitochondrial respiration and levels of CPT1A and cytokines (IL1B, IL6, IL8, IL10, TNF) in senescent cells. MT-RNR1 enhances mTORC2 activity while suppressing PTEN, promoting AKT phosphorylation and subsequent FOXO1 inhibition, reducing MSTN levels to stimulate skeletal muscle growth. Via the TGFB/SMAD pathway, it drives osteogenic differentiation of bone marrow mesenchymal stem cells and boosts osteoblast proliferation and type I collagen (COL1A1, COL1A2) synthesis.

Human

E. coli

N-His;N-GST

A0A0C5B5G6 (R2-R16)

/

Full Length of Mature Protein

Approximately 29.5 kDa

Greater than 90% as determined by reducing SDS-PAGE

Solution

Supplied as a 0.22 μm filtered solution of 50 mM HEPES (pH 7.5), 200mM NaCl, 20% glycerol, 1mM DTT.

<1 EU/μg, determined by LAL method.

Please use rapid thawing with running water to thaw the protein.

Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping with dry ice.