MRCKα Protein, Human (Sf9, GST)

The MRCKα protein is a key serine/threonine protein kinase downstream of CDC42 that coordinates cytoskeletal dynamics and cell migration. MRCKα phosphorylates PPP1R12C and MYL9/MLC2, regulating actin reorganization. MRCKα Protein, Human (Sf9, GST) is the recombinant human-derived MRCKα protein, expressed by Sf9 insect cells , with N-GST labeled tag.

MRCKα Protein, a serine/threonine-protein kinase, serves as a crucial downstream effector of CDC42, playing a pivotal role in orchestrating cytoskeleton reorganization and cell migration. Through phosphorylation of PPP1R12C and MYL9/MLC2, MRCKα actively regulates actin cytoskeletal reorganization. Teaming up with MYO18A and LURAP1, it contributes to the modulation of lamellar actomyosin retrograde flow, a process essential for cell protrusion and migration. MRCKα also phosphorylates PPP1R12A, LIMK1, and LIMK2, influencing various aspects of cellular dynamics. Furthermore, it may be involved in TFRC-mediated iron uptake and, in collaboration with FAM89B/LRAP25, facilitates the targeting of LIMK1 to the lamellipodium, leading to its activation and subsequent phosphorylation of CFL1—an event crucial for the regulation of lamellipodial F-actin. Additionally, MRCKα triggers the formation of an extrusion apical actin ring, playing a vital role in the epithelial extrusion of apoptotic cells.

Human

Sf9 insect cells

N-GST

Q5VT25 (S2-Q473)

8476

Greater than 90% as determined by reducing SDS-PAGE.

<1 EU/μg, determined by LAL method.