MMP-10 Protein, Human (HEK293)

MMP-10 protein is a member of the matrix metalloproteinase family and plays a crucial role in extracellular matrix remodeling by promoting the degradation of various substrates. As a protease, MMP-10 is involved in tissue homeostasis, wound healing, and inflammatory responses. MMP-10 Protein, Human (Active, HEK293) is the recombinant human-derived MMP-10 protein, expressed by HEK293, with tag free.

MMP-10, also known as Stromelysin-2 or transin-2, is classified into the simple hemopexin domain structural class. MMP-10 has a relatively broad substrate specificity, including proMMPs, ECM proteins, proteoglycans, glycoproteins, and collagens. Unlike several other MMPs that are localised predominantly in tumor stroma, tumor cells themselves appear to express MMP-10. MMP-10 is a potential tumor marker^[1].

Measured by its ability to cleave the fluorogenic peptide substrate, Mca­-RPKPVE-­Nval­-WRK(Dnp)-NH2 .The specific activity is >450 pmol/min/µg. (Activation description: The proenzyme needs to be activated by APMA (HY-148905) for an activated form.)

Human

HEK293

Tag Free

P09238 (Y18-C476)

4319

YPLSGAAKEEDSNKDLAQQYLEKYYNLEKDVKQFRRKDSNLIVKKIQGMQKFLGLEVTGKLDTDTLEVMRKPRCGVPDVGHFSSFPGMPKWRKTHLTYRIVNYTPDLPRDAVDSAIEKALKVWEEVTPLTFSRLYEGEADIMISFAVKEHGDFYSFDGPGHSLAHAYPPGPGLYGDIHFDDDEKWTEDASGTNLFLVAAHELGHSLGLFHSANTEALMYPLYNSFTELAQFRLSQDDVNGIQSLYGPPPASTEEPLVPTKSVPSGSEMPAKCDPALSFDAISTLRGEYLFFKDRYFWRRSHWNPEPEFHLISAFWPSLPSYLDAAYEVNSRDTVFIFKGNEFWAIRGNEVQAGYPRGIHTLGFPPTIRKIDAAVSDKEKKKTYFFAADKYWRFDENSQSMEQGFPRLIADDFPGVEPKVDAVLQAFGFFYFFSGSSQFEFDPNARMVTHILKSNSWLHC

Approximately 44 kDa & 55-60 kDa, based on SDS-PAGE under reducing conditions, due to the glycosylation.

Greater than 95% as determined by reducing SDS-PAGE.

Solution

Supplied as a 0.22 μm filtered solution of 50 mM Tris, 150 mM NaCl, pH 7.5 with 50% glycerol as protectant.
Note: For SPR assay, please replace the buffer. Primary amine components (e.g., Tris, imidazole) can affect protein-coupled chips.

<1 EU/μg, determined by LAL method.

Please use rapid thawing with running water to thaw the protein.

Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping with dry ice.

• [1]. Jason H Gill, et al. MMP-10 is overexpressed, proteolytically active, and a potential target for therapeutic intervention in human lung carcinomas. Neoplasia. 2004 Nov-Dec;6(6):777-85.  [Content Brief]