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  4. IRF9 Protein, Mouse

IRF9 Protein, Mouse is the recombinant mouse-derived IRF9 protein, expressed by E. coli, with tag free tag.

For research use only. We do not sell to patients.

Surface Plasmon Resonance (SPR) Assay Service   Protein Expression Service

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100 μg   Get quote  

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  • Biological Activity

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Description

IRF9 Protein, Mouse is the recombinant mouse-derived IRF9 protein, expressed by E. coli, with tag free tag.
Background

IRF9 is a transcription factor that plays an essential role in anti-viral immunity. It mediates signaling by type I IFNs (IFN-alpha and IFN-beta). Upon binding of type I IFNs to cell surface receptors, Jak kinases (TYK2 and JAK1) are activated, leading to tyrosine phosphorylation of STAT1 and STAT2. IRF9/ISGF3G then associates with the phosphorylated STAT1:STAT2 dimer to form the ISGF3 transcription factor complex, which translocates into the nucleus. ISGF3 binds to the interferon-stimulated response element (ISRE) to activate transcription of interferon-stimulated genes, thereby inducing an antiviral state in the cell.
Species

Mouse
Source

E. coli
Tag

Tag Free
Accession

Q61179 (M1-H399)
Gene ID

16391
Protein Length

Full Length
Synonyms
Isgf3g
Predicted Molecular Mass
44.7 kDa
Purity

Greater than 80% as determined by reducing SDS-PAGE
Endotoxin Level

<1 EU/μg, determined by LAL method.
Documentation
Handling Instructions (2659 KB)

IRF9 Protein, Mouse Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • Reconstitution Calculator

  • Dilution Calculator

  • Specific Activity Calculator

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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IRF9 Protein, Mouse
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