MGAT2 Protein, Human (HEK293, His)

The MGAT2 protein plays an important role in protein N-glycosylation, catalyzing the transfer of GlcNAc to the free terminal mannose in nascent N-linked glycan chains. This forms a second branch of the complex glycan and is critical for glycoprotein maturation and functional diversity. MGAT2 Protein, Human (HEK293, His) is the recombinant human-derived MGAT2 protein, expressed by HEK293 , with C-6*His labeled tag.

The MGAT2 protein assumes a fundamental role in protein N-glycosylation, facilitating a crucial step in the modification of nascent glycan chains. Specifically, MGAT2 catalyzes the transfer of N-acetylglucosamine (GlcNAc) onto the free terminal mannose moiety within the core structure of the nascent N-linked glycan chain. This enzymatic activity results in the formation of the second branch in complex glycans, a process essential for the proper maturation and functional diversity of glycoproteins. By mediating this glycosylation step, MGAT2 plays a central role in the intricate machinery governing the modification of proteins, contributing to cellular processes and functions crucial for overall biological activity.

Human

HEK293

C-6*His

Q10469 (R30-Q447)

4247  [NCBI]

Lumenal Domain

RQRKNEALAPPLLDAEPARGAGGRGGDHPSVAVGIRRVSNVSAASLVPAVPQPEADNLTLRYRSLVYQLNFDQTLRNVDKAGTWAPRELVLVVQVHNRPEYLRLLLDSLRKAQGIDNVLVIFSHDFWSTEINQLIAGVNFCPVLQVFFPFSIQLYPNEFPGSDPRDCPRDLPKNAALKLGCINAEYPDSFGHYREAKFSQTKHHWWWKLHFVWERVKILRDYAGLILFLEEDHYLAPDFYHVFKKMWKLKQQECPECDVLSLGTYSASRSFYGMADKVDVKTWKSTEHNMGLALTRNAYQKLIECTDTFCTYDDYNWDWTLQYLTVSCLPKFWKVLVPQIPRIFHAGDCGMHHKKTCRPSTQSAQIESLLNNNKQYMFPETLTISEKFTVVAISPPRKNGGWGDIRDHELCKSYRRLQ

Approximately 50.0 kDa

Greater than 95% as determined by reducing SDS-PAGE.

<1 EU/μg, determined by LAL method.