HMPV Protein, Human (HEK293, His)

Fusion glycoprotein F0 is cleaved to generate mature F1 and F2 fusion glycoproteins. It transitions through different conformational states to promote the fusion of virus and cell membranes. HMPV Protein, Human (HEK293, His) is the recombinant virus-derived HMPV protein, expressed by HEK293, with C-His labeled tag.

The Fusion glycoprotein F0 is an inactive precursor undergoing cleavage to generate the mature F1 and F2 fusion glycoproteins, representing a class I viral fusion protein. According to the current model, the protein exhibits at least three conformational states: the pre-fusion native state, pre-hairpin intermediate state, and post-fusion hairpin state. In viral and plasma cell membrane fusion events, the coiled coil regions adopt a trimer-of-hairpins structure, aligning the fusion peptide in close proximity to the C-terminal region of the ectodomain. The formation of this structure drives apposition and subsequent fusion of viral and cellular membranes, facilitating the delivery of the nucleocapsid into the cytoplasm. Remarkably, this fusion process is pH independent and occurs at either the plasma or endosomal membrane. The trimer of F1-F2 (F protein) also plays a critical role in host cell attachment by binding to host heparan sulfate, highlighting its multifaceted role in mediating viral entry and infection.

Virus

HEK293

C-His

Q6WB98 (L19-T489)

/

Partial

Approximately 70.2 kDa & 50.5 kDa & 17.2 kDa,based on SDS-PAGE under reducing conditions.

Greater than 90% as determined by reducing SDS-PAGE.

Lyophilized powder.

Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.