1. Recombinant Proteins
  2. Complement System
  3. MASP-2
  4. MASP2 Protein, Cynomolgus (His)

MASP2 Protein exhibits a deficiency in conserved residue(s) crucial for feature annotation propagation. MASP2 Protein, Cynomolgus (His) is the recombinant cynomolgus-derived MASP2 protein, expressed by E. coli , with C-His labeled tag.

For research use only. We do not sell to patients.

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  • Biological Activity

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Description

MASP2 Protein exhibits a deficiency in conserved residue(s) crucial for feature annotation propagation. MASP2 Protein, Cynomolgus (His) is the recombinant cynomolgus-derived MASP2 protein, expressed by E. coli , with C-His labeled tag.

Background

The MASP2 protein is characterized by a deficiency in conserved residue(s) essential for the propagation of feature annotation.

Biological Activity

Immobilized Cynomolgus MASP2, His Tag at 1μg/ml (100μl/well) on the plate. Dose response curve for Anti-MASP2 Antibody, hFc Tag with the EC50 of 11.9 ng/ml determined by ELISA.

Species

Cynomolgus

Source

E. coli

Tag

C-6*His

Accession

A0A2K5UJY0 (T287-F686)

Gene ID
Molecular Construction
N-term
MASP2 (T287-F686)
Accession # A0A2K5UJY0
6*His
C-term
Protein Length

Partial

Synonyms
MAP19; MASP-2; MASP1P1; sMap
Molecular Weight

Apparent Molecular weight is 21-24 kDa&28-30 kDa

Purity

Greater than 95% as determined by Tris-Bis PAGE

Appearance

Lyophilized powder

Formulation

Lyophilized from a 0.2 μm filtered solution of 50mM Tris, 200mM NaCl (pH 9.0). Normally 8% trehalose / 8% mannitol is added as protectant before lyophilization.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O.

Storage & Stability

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US; may vary elsewhere.

Documentation

MASP2 Protein, Cynomolgus (His) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • Reconstitution Calculator

  • Dilution Calculator

  • Specific Activity Calculator

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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Product Name:
MASP2 Protein, Cynomolgus (His)
Cat. No.:
HY-P77799
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