2. Recombinant Proteins
  3. Complement System
  4. MASP-1
  5. MASP1 Protein, Mouse (HEK293, His)

MASP1 Protein, Mouse (HEK293, His) is the recombinant mouse-derived MASP1 protein, expressed by HEK293, with C-His tag.

For research use only. We do not sell to patients.

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Description

MASP1 Protein, Mouse (HEK293, His) is the recombinant mouse-derived MASP1 protein, expressed by HEK293, with C-His tag.
Background

MASP1 functions in the lectin pathway of complement, which plays a key role in innate immunity by recognizing pathogens through sugar moiety patterns. The pathway is triggered when mannan-binding lectin (MBL) and ficolins bind to sugar moieties, leading to activation of associated proteases MASP1 and MASP2. It acts as an endopeptidase and may activate MASP2 or C2, or directly activate C3, the central component of the complement cascade. Isoform 2 may inhibit lectin pathway activation or cleave IGFBP5. MASP1 also contributes to developmental processes.
Species

Mouse
Source

HEK293
Tag

C-His
Accession

P98064-2 (I455-R733)
Gene ID

17174
Molecular Construction
N-term
MASP1 (I455-R733)
Accession # P98064-2
His
C-term
Protein Length

Partial
Synonyms
MASP3; MASP1; CRARF1; RaRF; Mannan-binding lectin serine protease 1; Complement factor MASP-3; Complement-activating component of Ra-reactive factor; Mannose-binding lectin-associated serine protease 1 (MASP-1); Mannose-binding protein-associated serine protease; Ra-reactive factor serine protease p100 (RaRF); Serine protease 5
AA Sequence

IGGRNAELGLFPWQALIVVEDTSRVPNDKWFGSGALLSESWILTAAHVLRSQRRDNTVIPVSKEHVTVYLGLHDVRDKSGAVNSSAARVILHPDFNIQNYNHDIALVQLQKPVPLGAHVMPICLPRPEPEGPAPHMLGLVAGWGISNPNVTVDEIILSGTRTLSDVLQYVKLPVVSHAECKASYESRSGNYSVTENMFCAGYYEGGKDTCLGDSGGAFVIFDEMSQHWVAQGLVSWGGPEECGSKQVYGVYTKVSNYVDWLWEEMNSPRAVRDLQVER

Predicted Molecular Mass
32.6 kDa
Molecular Weight

Approximately 40-45 kDa,based on SDS-PAGE under reducing conditions,due to the glycosylation.
Purity

Greater than 95% as determined by reducing SDS-PAGE.
Endotoxin Level

<1 EU/μg, determined by LAL method.
Documentation
SDS (251 KB)

COA (220 KB)

Handling Instructions (2659 KB)

MASP1 Protein, Mouse (HEK293, His) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • Reconstitution Calculator

  • Dilution Calculator

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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