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  4. MARCHF1 Protein, Human (His)

MARCHF1 Protein, Human (His) is the recombinant human-derived MARCHF1 protein, expressed by E. coli, with N-10*His tag.

For research use only. We do not sell to patients.

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Description

MARCHF1 Protein, Human (His) is the recombinant human-derived MARCHF1 protein, expressed by E. coli, with N-10*His tag.
Background

MARCHF1 is an E3 ubiquitin-protein ligase that mediates ubiquitination of TFRC, CD86, FAS, and MHC class II proteins, such as HLA-DR alpha and beta, promoting their endocytosis and sorting to lysosomes via multivesicular bodies. In immature dendritic cells, it constitutively ubiquitinates MHC class II proteins, downregulating their cell surface localization and sequestering them in the intracellular endosomal system. Additionally, MARCHF1 regulates insulin sensitivity by controlling the surface expression of the insulin receptor subunit beta/INSR through direct ubiquitination and degradation.
Species

Human
Source

E. coli
Tag

N-10*His
Accession

Q8TCQ1 (M1-V289)
Gene ID

55016
Molecular Construction
N-term
10*His
MARCHF1 (M1-V289)
Accession # Q8TCQ1
C-term
Protein Length

Full Length
Synonyms
MARCHF1; MARCH1; RNF171; E3 ubiquitin-protein ligase MARCHF1; Membrane-associated RING finger protein 1; Membrane-associated RING-CH protein I; MARCH-I; RING finger protein 171; RING-type E3 ubiquitin transferase MARCHF1
AA Sequence

MLGWCEAIARNPHRIPNNTRTPEISGDLADASQTSTLNEKSPGRSASRSSNISKASSPTTGTAPRSQSRLSVCPSTQDICRICHCEGDEESPLITPCRCTGTLRFVHQSCLHQWIKSSDTRCCELCKYDFIMETKLKPLRKWEKLQMTTSERRKIFCSVTFHVIAITCVVWSLYVLIDRTAEEIKQGNDNGVLEWPFWTKLVVVAIGFTGGLVFMYVQCKVYVQLWRRLKAYNRVIFVQNCPDTAKKLEKNFSCNVNTDIKDAVVVPVPQTGANSLPSAEGGPPEVVSV

Predicted Molecular Mass
32.3 kDa
Purity

Greater than 85% as determined by reducing SDS-PAGE.
Endotoxin Level

<1 EU/μg, determined by LAL method.
Documentation
Handling Instructions (2659 KB)

MARCHF1 Protein, Human (His) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • Reconstitution Calculator

  • Dilution Calculator

  • Specific Activity Calculator

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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MARCHF1 Protein, Human (His)
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