MAOA Protein, Human (Active, sf9, His)

MAOA Protein, Human (Active, sf9, His) is the recombinant human-derived MAOA protein, expressed by Sf9 insect cells, with N-His tag.

Monoamine Oxidase A (MAO-A) catalyzes the oxidative deamination of primary and some secondary amines, such as neurotransmitters, while reducing oxygen to hydrogen peroxide. It plays a crucial role in metabolizing neuroactive and vasoactive amines in the central nervous system and peripheral tissues. MAO-A preferentially oxidizes serotonin. Additionally, it catalyzes the oxidative deamination of kynuramine to form 3-(2-aminophenyl)-3-oxopropanal, which can spontaneously condense into 4-hydroxyquinoline (by similar).

Measured by its ability to produce hydrogen peroxide during the oxidation of Tyramine. The specific activity is >300 pmol/min/ug.

Human

Sf9 insect cells

N-His

P21397-1 (E2-S527)

4128

Partial

ENQEKASIAGHMFDVVVIGGGISGLSAAKLLTEYGVSVLVLEARDRVGGRTYTIRNEHVDYVDVGGAYVGPTQNRILRLSKELGIETYKVNVSERLVQYVKGKTYPFRGAFPPVWNPIAYLDYNNLWRTIDNMGKEIPTDAPWEAQHADKWDKMTMKELIDKICWTKTARRFAYLFVNINVTSEPHEVSALWFLWYVKQCGGTTRIFSVTNGGQERKFVGGSGQVSERIMDLLGDQVKLNHPVTHVDQSSDNIIIETLNHEHYECKYVINAIPPTLTAKIHFRPELPAERNQLIQRLPMGAVIKCMMYYKEAFWKKKDYCGCMIIEDEDAPISITLDDTKPDGSLPAIMGFILARKADRLAKLHKEIRKKKICELYAKVLGSQEALHPVHYEEKNWCEEQYSGGCYTAYFPPGIMTQYGRVIRQPVGRIFFAGTETATKWSGYMEGAVEAGERAAREVLNGLGKVTEKDIWVQEPESKDVPAVEITHTFWERNLPSVSGLLKIIGFSTSVTALGFVLYKYKLLPRS

Approximately 60 kDa,based on SDS-PAGE under reducing conditions.

Greater than 95% as determined by reducing SDS-PAGE.

Solution

Supplied as a 0.2 μm filtered solution of 50 mM HEPES, 150 mM NaCl, DDM, CHS, pH 7.5 with 50% glycerol as protectant.

<1 EU/μg, determined by LAL method.

N/A.

Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping with dry ice.