MAOA Protein, Human (Active, sf9, His)

MAOA Protein, Human (Active, sf9, His) is the recombinant human-derived MAOA protein, expressed by Sf9 insect cells, with N-His tag. This product contains detergent and is not suitable for cell-related experiments.

Monoamine Oxidase A (MAO-A) catalyzes the oxidative deamination of primary and some secondary amines, such as neurotransmitters, while reducing oxygen to hydrogen peroxide. It plays a crucial role in metabolizing neuroactive and vasoactive amines in the central nervous system and peripheral tissues. MAO-A preferentially oxidizes serotonin. Additionally, it catalyzes the oxidative deamination of kynuramine to form 3-(2-aminophenyl)-3-oxopropanal, which can spontaneously condense into 4-hydroxyquinoline (by similar).

Measured by its ability to produce hydrogen peroxide during the oxidation of Tyramine. The specific activity is >300 pmol/min/ug.

Human

Sf9 insect cells

N-His

P21397-1 (E2-S527)

4128

Partial

ENQEKASIAGHMFDVVVIGGGISGLSAAKLLTEYGVSVLVLEARDRVGGRTYTIRNEHVDYVDVGGAYVGPTQNRILRLSKELGIETYKVNVSERLVQYVKGKTYPFRGAFPPVWNPIAYLDYNNLWRTIDNMGKEIPTDAPWEAQHADKWDKMTMKELIDKICWTKTARRFAYLFVNINVTSEPHEVSALWFLWYVKQCGGTTRIFSVTNGGQERKFVGGSGQVSERIMDLLGDQVKLNHPVTHVDQSSDNIIIETLNHEHYECKYVINAIPPTLTAKIHFRPELPAERNQLIQRLPMGAVIKCMMYYKEAFWKKKDYCGCMIIEDEDAPISITLDDTKPDGSLPAIMGFILARKADRLAKLHKEIRKKKICELYAKVLGSQEALHPVHYEEKNWCEEQYSGGCYTAYFPPGIMTQYGRVIRQPVGRIFFAGTETATKWSGYMEGAVEAGERAAREVLNGLGKVTEKDIWVQEPESKDVPAVEITHTFWERNLPSVSGLLKIIGFSTSVTALGFVLYKYKLLPRS

Approximately 60 kDa, based on SDS-PAGE under reducing conditions.

Greater than 95% as determined by reducing SDS-PAGE.

Solution

Supplied as 0.2 μm filtered solution of 50 mM HEPES, 150 mM NaCl, DDM, CHS, pH7.5 with glycerol as protectant. Due to the cytotoxicity of the detergent, this product is not appropriate for cell-based experiments.

<1 EU/μg, determined by LAL method.

N/A.

Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping with dry ice.